摘要
目的 :探讨普萘洛尔对去卵巢大鼠骨质疏松症的防治作用以及对血清瘦素水平的影响。方法 :5 0只健康雌性未交配 6个月龄SD大鼠以切除卵巢复制绝经后骨质疏松模型 ,分为 5组 :①假手术组 (Sham) ;②雌激素治疗组 (O +E) ;③普萘洛尔组 (O +P) ;④雌激素 +普萘洛尔组 (O +E +P) ;⑤安慰剂组 (OVX) ;OVX后 1周开始经灌胃给予普萘洛尔每天 8mg·kg 1·d 1、皮下注射雌激素 2 0 μg·kg 1·d 1,共 12周。动物处死时留取第 3、4、5腰椎、血清标本。进行骨密度分析、血清瘦素水平及体重测定。结果 :O +P、O +P +E和O +E组的骨密度较安慰剂 (OVX)组明显增高 (P <0 .0 5 ) ,O +P组血清瘦素水平较OVX组明显升高 (P <0 .0 5 ) ,O +P +E组较O +E组和Sham组明显升高 (P <0 .0 1)。结论 :普萘洛尔能预防卵巢切除术后大鼠骨丢失 ,预防OVX大鼠的骨质疏松症 。
Objective:To investigative the effect of propranolol on body mineral density(BMD) and serum leptin in rats induced by ovariectomy(OVX). Methods: Fifty virgin female SD rats of six months old were divided into five groups: (1) Sham;(2) OVX+ 17β- estradial 20μg·kg -1 ·d -1 SC(O+E);(3) OVX+ Propranolol 8mg·kg -1 ·d -1 (O+P);(4)O+E+P (5) OVX. The administration of drugs were started one week after operation and continued for 12 weeks. Blood samples were collected before sacrifice to determine serum Leptin. The lumbar vertebrae were collected for BMD analysis. Results:Treatment of OVX rats with propranolol (O+P) increased BMD. These changes were similar to those 17β-estradial treatment group (O+E). Leptin increased in O+P group and O+P+E group,their body weight were similar to OVX group and O+E group. Conclusion:Propranolol prevent bone loss and increase serum leptin in OVX rats.
出处
《军医进修学院学报》
CAS
2004年第3期179-181,共3页
Academic Journal of Pla Postgraduate Medical School
基金
国家卫生部默沙东基金资助项目 (19980 612 )
