脂多糖对感染卡介苗巨噬细胞表达细胞因子影响的研究

Cytokine expression of lipopolysaccharide-stimulated macrophage infected by M.bovis BCG

目的 探讨脂多糖 (LPS)对感染卡介苗巨噬细胞表达细胞因子的影响。方法 用ELISA方法比较单独巨噬细胞组 ,巨噬细胞 +卡介苗 +LPS组 ,巨噬细胞 +卡介苗组以及巨噬细胞 +LPS组细胞因子表达的差异。结果 单纯巨噬细胞组产生较少量的IL 12 ,TNF α ,LPS能刺激巨噬细胞组特别是感染卡介苗巨噬细胞组产生大量的IL 12 ,TNF α。与单纯巨噬细胞组相比 ,LPS也不能有效地刺激巨噬细胞产生更多的IL 1,IL 2 ,IL 18和IFN γ。结论 LPS能诱导巨噬细胞 (包括卡介苗感染巨噬细胞 )产生更多的TNF α和IL 12 ,而有利于宿主的免疫应答。

Objective To study whether lipopolysaccharide (LPS) can influence cytokine expression of macrophage infected by M.bovis BCG.Methods ELISA method was used to measure the cytokine expression of the different groups including macrophage group, macrophage +BCG+LPS group,macrophage +BCG group, macrophage +LPS group, and thus to compare the differences.Results Little IL-12 and TNF-α was produced in single macrophage group,LPS can stimulate macrophage group especially macrophage group infected by M.bovis BCG to produce significant amount of IL-12 and TNF-α.Compared with single macrophage group,LPS also can't stimulate macrophage group including macrophage group infected by M.bovis BCG to produce more IL-1,IL-2,IL-18 and IFN-γ.Conclusion LPS can stimulate macrophage to produce more IL-12,TNF-α.That is conducive to the host immune response.It is suggested that LPS and its analogues may have potential for anti-TB drugs,vaccines or their adjuvants.