精索静脉曲张大鼠睾丸一氧化氮合酶的表达与生精功能损害关系研究

The nitrous oxide synthase expression and spermatogenesis defect in varicocele testis of rats

目的探索精索静脉曲张大鼠睾丸一氧化氮合酶NOS表达的改变及其对生精功能的影响。方法选择30例SD大鼠,20例作为实验组,建立精索静脉曲张模型;10例作为假手术对照组。3个月后,取睾丸常规切片染色,用Makler积分法分别测量实验组200个、假手术对照组100个曲细精管的生精功能,并计算平均得分;免疫组化方法测定NOS的表达强度,并比较两组之间的差异。结果实验组Makler评分显著低于假手术对照组8.5±0.6)分vs(16.0±1.2)分,差异有非常显著性意义t=-6.583P<0.001)。实验组曲细精管NOS表达强度为3.74±0.37,假手术对照组表达强度为2.10±0.12,差异有非常显著性意义(t=-6.586,P<0.001),且其表达强度与评分值呈负相关(r=-0.92P<0.01。结论精索静脉曲张睾丸组织中NOS表达的增加可能与生精功能损害有关,并影响精子细胞的成熟。

Objective To investigate the relationship of nitrous oxide synthase (NOS) and the spermatogenesis defect in varicocele testis of rats. Methods Twenty rats were operated as the varicocele group and other 10 were sham-operated, as the control group, testes were removed, stained and examined pathologically. Makler Score was adopted to analyze the mean score in 200 and 100 seminiferous tubulae respectively. NOS was determined by immunohistochemistry, the differences between two groups were compared. Results In varicocele group, the mean score of seminiferous tubule was lower than that of control group (8.5±0.6 vs 16.0±1.2,P<0.001). NOS expression was higher in varicocele group than that in controls (3.74±0.37vs2.10±0.12,P<0.001).The two parameters were negatively correlated (r=-0.92, P<0.01). Conclusions NOS overexpression in varicocele testis could lead to the damage of spermatogenesis and spermatic immaturity.