经肝动脉灌注三氧化二砷对肝移植瘤血管的作用

Effects of arsenic trioxide (As_2O_3) by transcatheter arterial infusion on vessel of hepatic Vx-2 carcinoma in rabbits

目的 :探讨经肝动脉灌注As2 O3 对肝移植瘤血管的作用 .方法 :采用新西兰大白兔建立 5 0只肝脏Vx 2移植瘤动物模型 ,随机平均分成 5组 .移植瘤术后 2wk ,经肝动脉插管灌注不同浓度As2 O3 ,并以顺铂和生理盐水作对照 ,连续 7d ,6wk末切取肝肿瘤组织 ,观察肿瘤组织微血管有无改变 ,检测移植瘤组织血管内皮生长因子 (VEGF)的表达 .结果 :实验组肿瘤组织毛细血管内皮细胞肿胀、细胞核碎裂、细胞坏死及微血栓形成 ,阴性对照组和阳性对照组无类似改变 .实验组VEGF表达低于对照组 ,差异有显著性 (P <0 .0 1 ) ,而实验组间VEGF表达无差异性 (P =1 .0 0 ) .结论 :经肝动脉灌注As2 O3可减少肿瘤组织的血管生成 ;选择性破坏肝移植瘤组织血管 。

AIM: To investigate the effects of arsenic trioxide (As2O3) by transcatheter arterial infusion (TAI) on the vessel of hepatic Vx 2 carcinoma in rabbits. METHODS: Fifty New Zealand rabbits weighing 2 3 kg with hepatic Vx 2 carcinoma were randomly divided into five groups two weeks after implantation and were treated with As2O3 (experimental groups), cisplatin or normal saline (control groups ) by TAI for 7 consecutive days. Three weeks later, the capillary endothelial cells of tumor tissues were investigated under transmission electron microscope and vascular endothelial cell growth factor (VEGF) expressions were examined by immunohistochemistry. RESULTS: The swelling and necrosis of capillary endothelial cells, and the microthrombus in tumor tissue were observed under transmission electron microscope in the experimental groups. Significantly decreased VEGF expression was also observed in As2O3 groups in comparison with that in the control groups ( P <0.01). CONCLUSION: As2O3 by transcatheter arterial infusion can reduce the generation of blood vessels and can selectively damage the blood vessels of tumor tissues. The effect of As2O3 by TAI is in a dose dependent manner.