苦参碱诱导K562细胞分化早期的基因表达分析

Gene expression profile in early differentiation of K562 cells induced by matrine

目的 比较苦参碱诱导K5 6 2细胞前后的基因表达谱 ,寻找与肿瘤细胞分化相关的基因。方法 0 .1mg/ml苦参碱作用K5 6 2细胞 3h后 ,采用基因芯片技术测定并分析对照组与苦参碱处理组间的基因表达谱差异。结果 在 84 6 5个候选基因中 ,筛选出 30个差异表达基因 ,苦参碱处理组与对照组比较 ,表达上调的基因有 12个 ,主要涉及代谢相关蛋白基因、细胞受体等 ;表达下调的有18个 ,包括抑癌基因、原癌基因、DNA结合与转录因子、代谢相关蛋白基因、细胞信号转导基因等。结论 肿瘤细胞诱导分化是多基因作用的综合结果 ,筛选的基因对了解肿瘤发生、发展与逆转分化 ,以及寻找潜在的药物作用靶点可能有意义。

Objective To compare gene expression profile in K562 cells induced by matrine, so as to screen for diffentiation related genes. Methods K562 cells were exposed to 0.1 mg/ml matrine for 3 hours, and gene expression profiles in matrine-treated and non-treated cells were studied by cDNA microarray. Results From 8465 screened genes, 30 differentially expressed genes were found.Among them 18 showed decreased expression including oncogene and proto-oncogene,signal transduction gene,DNA binding and transcription gene etc,12 showed increased expression including cell receptor gene,immune-associated gene,metabolism-associated gene etc in matrine-treated K562 cells as compared with that in non-treated cell. Conclusion The genes,that are closely associated with differentiation of can-cer cell, could be the potential targets for cancer treatment.