川芎嗪对兔颈动脉球囊损伤后内膜增生的影响

Effect of ligustrazine on intimal proliferation in balloon-injured rabbit carotid artery

目的 研究川芎嗪对兔颈动脉球囊损伤后新生内膜及管腔狭窄度的影响 ,探讨其预防再狭窄的可能性。方法 应用直径为 3mm的球囊导管造成家兔左颈动脉去内皮损伤模型。术后 14d和 2 8d分别测量对照组和川芎嗪治疗组动脉新生内膜及中膜厚度、新生内膜面积和管腔狭窄度并应用Brdu和α actin免疫组化方法观察新生内膜细胞的增殖指数。结果 治疗组新生内膜及中膜厚度、新生内膜面积、管腔狭窄度和细胞增殖指数均明显低于对照组。结论 川芎嗪具有明显抑制动脉损伤后新内膜的形成及降低管腔狭窄度的作用。

Objective To study the influence of ligustrazine on neointima and stenotic rate of arterial lumen in rabbit carotid artery after balloon injury for evaluating the possibility of ligustrazine on the prevention of restenosis. Methods Artery injury models in deendothelialization of the left carotid artery of rabbits were completed by using balloon catheter 3mm in diameter. Neointimal thickness,medial thickness,neointimal area and stenotic ratio of arterial lumen were measured and proliferation index of neointimal cells were observed by the method of Brdu and α-actin inmmunohistochemistry between drug groups and control groups,and their difference was compared at 14d and 28d after balloon injury. Results Treatment regimen produced significant reductions in neointimal thickness,medial thickness,neointimal area,stenotic rate of arterial lumen and proliferation index of cells in neointima. Conclusion Ligustrazine has a significant role of inhibiting neointimal formation and reducing stenotic ratio of arterial lumen after balloon injury.