磷酸化cAMP反应元件结合蛋白在全脑缺血大鼠海马中的表达

The expression of phospho-cAMP response element binding protein in hippocampus of rats reperfusion following global ischemia

目的 观察转录因子磷酸化cAMP反应元件结合蛋白 (pCREB)在大鼠短暂性全脑缺血再灌注后海马中的表达 ,并探讨其表达的意义。方法 采用四动脉阻断法制备大鼠全脑缺血模型 ,缺血 15min。Western印迹检测海马组织pCREB的表达 ,免疫组化观察其在大鼠海马CA1区及齿状回的表达。结果 Western印迹显示 ,缺血再灌注 3h ,pCREB的表达至高峰 ,并随再灌注时间延长呈现下降趋势。免疫组化显示缺血再灌注 3h海马CA1区pCREB表达至高峰 ,并迅速下降 ,于 2 4h降至假手术组水平 ;而在齿状回其表达较强且持久。结论 脑缺血再灌注促进pCREB的表达 ,pCREB的表达可能参与了缺血性脑损伤的内源性保护机制。

Objective To explore the expression of pCREB in hippocampus of rats reperfusion following global ischemia. Methods Adult male Sprague-Dawley (SD)rats were subjected to the 15min transient cerebral ischemia induced by the four-vessel occlusion method. Using Western Blotting and immunohistochemistry with anti-phospho-CREB antibody,we studied that hippocampd neurons' injury,changes of pCREB content and its distribution. Results Immunohistochemistry staining manifested the number of pCREB positive cells in the I/R group reached the maximum at 3h and declined quickly to the Sham group level at 24h in the CA 1 subfield. While there were stronger and longer expressions in the DG subfield. Western Blot analysis showed pCREB expression level reached the peak at 3h in I/R group,and decreased in a time-dependent manner. Conclusion Global ischemia-reperfusion promotes the expression of pCREB,which maybe involved in protection after cerebral ischemia.