成骨细胞株与强韧化纳米人工骨支架联合培养实验研究

Experimental study on union culture of osteoblast-like cells(3T3-E1)with hyper-porosity Ca_3(PO_4)_2 bone cells scaffold/NMZrO_2 reinforced in vitro

目的 :观察成骨细胞株 ( 3T3 E1)在新型纳米氧化锆强韧化高孔隙率人工骨支架 (下文简称人工骨支架 )材料上生长情况。方法 :成骨细胞株 ( 3T3 -E1)与人工骨支架联合培养 ,通过光镜观察和细胞计数的方法了解成骨细胞与支架结合能力 ,扫描电镜观察细胞生长的情况。结果 :新型强韧化纳米人工骨材料与建株成骨细胞有良好的结合能力 ,成骨细胞株 ( 3T3 E1)在人工骨材料上生长良好。结论 :纳米骨支架是较理想的骨组织工程支架材料 ,成骨细胞复合支架用于骨缺损的修复 ,具有广阔的临床应用前景。

Objective:To investigate the growth state of osteoblast-like cells(3T3-E1)on hyper-porosity Ca_3(PO_4)_2 bone cells scaffold/NM ZrO_2 reinforced(be called scaffold for short in the following text)in vitro.Method:The cells were union cultured on the scaffold.The growth state and combination ability of osteoblast-like cells with scaffold was observed by optical microscope,scaning electron microscope and cells counting respectively.Result:osteoblast-like cells(3T3-E1)grew well on the scaffold.New type scaffold had good combination ability with cells,and showed no adverse effect on cell function.Conclusion:New type scaffold is an optimal scaffold material for bone tissue engineering,which may have potentially clinical application for bone defect repair.