生长抑素增强阿霉素杀伤胆囊癌细胞的作用

Somatostatin enhances antitumor effect of doxorubicin on gallbladder caner cell

目的探讨阿霉素在单独用药及与生长抑素联合用药时,对离体胆囊癌细胞生长的影响。方法生长抑素和细胞增殖期特异性化疗药物阿霉素,作用于体外培养的人胆囊癌细胞株GBC-SD,通过阿霉素半数抑癌浓度值(IC50)的变化,观察生长抑素对其杀伤癌细胞能力的影响;并应用流式细胞仪观察联合用药在不同时间点胆囊癌细胞周期以及凋亡率的变化,与单独使用化疗药物的癌细胞周期和生长情况相比较。结果生长抑素能够降低阿霉素对胆囊癌细胞的IC50值,其作用呈浓度-效应依赖性(P<0.05)。单独用药与联合用药均能使得癌细胞生长阻滞于细胞周期的S期,但后者于作用72 h后诱导细胞凋亡作用强于前者。结论生长抑素可增强胆囊癌细胞对化疗药物阿霉素的敏感性,可能降低阿霉素的化疗毒副作用。其机制可能在于联合用药诱导细胞生长停滞和细胞凋亡。

Objective To explore the antitumor effect of doxorubicin on gallbladder cancer cell growth when used alone or combined with somatostatin. Methods Fifty percent of gallbladder cancer cell viability concentration (IC50) of doxorubicin in the absence or presence of somatostatin was measured. Then, the change of gallbladder cancer cell cycle and apoptosis index were studied using flow cytometry after treatment by doxorubicin alone or combined with somatostatin. Results Concentration-dependent down-regulation of IC50 of doxorubicin was detected by somatostatin (P < 0. 05 ) . Cell growth arrest in S phase was found by doxorubicin combined with somatostatin as well as using doxorubicin alone. While 72 h after treatment with doxorubicin combined with somatostatin, apoptosis index increased significantly than that of doxorubicin using alone. Conclusion Somatostatin can enhance chemosensitivity of Doxorubicin on gallbladder cancer cells. It might result from inducing growth arrest in S phase and apoptosis.