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PTEN基因对子宫内膜癌细胞生长的抑制作用 被引量：7

Inhibitory effect of tumor suppressor PTEN on cell growth of endometrial carcinoma
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摘要 目的　探讨PTEN基因在子宫内膜癌基因治疗方面的可行性。方法　通过腺病毒载体(Ad PTEN)将人野生型PTENcDNA导入Ishikawa细胞 ,观察外源性PTEN蛋白的表达情况 ,以及PTEN蛋白对Ishikawa细胞生长的影响 ,同时还观察了PTEN蛋白是否影响胰岛素样生长因子Ⅱ (IGF Ⅱ )对Ishikawa细胞的作用。结果　Ad PTEN感染Ishikawa细胞后 1d ,即可见PTEN蛋白表达 ;第 3天时 ,表达明显增强 ,并能持续表达 10d。感染后的Ishikawa细胞生长受到明显抑制 ,并且PTEN还能明显抑制IGF Ⅱ诱导的Ishikawa细胞生长。结论　腺病毒介导的PTEN基因导入能诱导Ishikawa细胞生长受到明显抑制 ,提示野生型PTEN基因可能是治疗子宫内膜癌的一种新途径。 Objective To evaluate the inhibitory effect of tumor suppressor PTEN on cell growth of endometrial carcinoma. Methods The exogenous wild PTEN cDNA via an adenoviral vector (Ad-PTEN) was introduced into Ishikawa cells. The expression of PTEN protein was detected by Western blot. The growth of Ishikawa cells was evaluated by trypan blue exclusion method and MTT. Results The expression of PTEN protein was induced on day 1, and greatly increasing on day 3-5 after Ad-PTEN infection. The expression of PTEN significantly inhibited the growth of Ishikawa cells, and also significantly inhibited the growth of Ishikawa cells induced by IGF-Ⅱ. Conclusion Adenovirus-mediated introduction of exogenous PTEN into human endometrial carcinoma cells can induce growth suppression. PTEN gene may be a novel therapeutic agent for endometrial carcinoma.
作者 万小云 石一复 李娟清
机构地区 浙江大学医学院附属妇产科医院
出处 《中华肿瘤杂志》 CAS CSCD 北大核心 2004年第5期275-278,共4页 Chinese Journal of Oncology
关键词 PTEN基因 子宫内膜癌 癌细胞 细胞生长 抑制作用 基因治疗 PTEN gene Endometrial neoplasms IGF-Ⅱ
分类号 R737.33 [医药卫生—肿瘤]
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参考文献1

二级参考文献10

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共引文献6

同被引文献45

  • 1Ye-JiangZhou,Yu-XiaXiong,Xiao-TingWu,DeShi,WeiFan,TongZhou,Yue-ChunLi,XiongHuang.Inactivation of PTEN is associated with increased angiogenesis and VEGF overexpression in gastric cancer[J].World Journal of Gastroenterology,2004,10(21):3225-3229. 被引量：31
  • 2吕庆杰,赵晓东,宋继谒,李晓晗,马颖,孟辉,姜卫国.卵巢癌PTEN基因失活机制的探讨[J].中华病理学杂志,2005,34(5):266-269. 被引量：15
  • 3Li J, Yen C, Liaw D, et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science, 1997,275:1943-1947.
  • 4Steck PA, Pershouse MA, SA, et al. Identification of a candidate tumor suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat Genet, 1997,15:356-362.
  • 5Tashiro H, Blazes MS, Wu R, et M. Mutations in PTEN are quent in endometrial carcinoma but rare in other common gynecological malignancies. Cancer Res. 1997,57:3935-3940.
  • 6Risinger JI, Hayes AK, Berchuck A, et al. PTEN/MMAC1 mutations in endometrial cancers. Cancer Res, 1997,57: 4736-4738.
  • 7Maxwell GL, Risinger JI, Gumbs C, et al. Mutation of the PTEN tumor suppressor gene in endometrial hyperplasias. Cancer Res,1998,58 : 2500-2503.
  • 8Wan X. Yokoyama Y, Shinohara A, et al. PTEN augments staurosporine-induced apoptosis in PTEN-null lshikawa cells by down regulating PI3K/Akt signaling pathway. Cell Death Differ, 2002,9:414-420.
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  • 10Chu EC, Tarnawski AS. PTEN regulatory functions in tumor suppression and cell biology. Med Sci Monit, 2004, 10: RA235-241.

引证文献7

二级引证文献50

中华肿瘤杂志
2004年 第5期

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