NAT2基因多态性与膀胱癌遗传易感性的关系

Relationship between genetic polymorphism of NAT2 and susceptibility to urinary bladder cancer

目的 探讨NAT2基因多态性与膀胱癌遗传易感性的关系。方法 以病例对照研究方法 ,采用PCR RFLP技术 ,对 6 9例膀胱癌患者和 88例健康对照者的NAT2基因型进行检测 ,分析NAT2基因型的分布特征。结果 NAT2慢基因型在病例组的频率为 2 6 .1% (18/6 9) ,对照组为 14 .8% (13/88) ,差异有显著性 (P <0 .0 5 ) ,携带慢基因型者患膀胱癌的危险性比携带快基因型者高 2倍。以NAT2快基因型 /不吸烟者为参照 ,慢基因型 /吸烟者患膀胱癌的危险性增加 5 .8倍 (P <0 .0 5 )。在吸烟量 >10PY(1PY =平均每年每天吸烟 2 0支 )的吸烟者中 ,病例组的慢基因型分布频率显著高于对照组 (P <0 .0 5 )。病例组中 ,NAT2基因型与膀胱癌组织分级呈显著相关性 (P <0 .0 5 )。慢基因型在表浅性肿瘤患者中的频率为 12 .8% ,浸润性肿瘤患者则为 5 4 .5 % ,差异有显著性 (P <0 .0 1)。结论NAT2基因多态性与膀胱癌的易感性有关 ,携带慢基因型的人群易患膀胱癌 ,而且肿瘤恶性程度更高。

Objective To study the relationship between genetic polymorphism of NAT2 and susceptibility to bladder cancer. Methods NAT2 genotypes were determined by PCR-RFLP method in 69 patients with bladder transitional cell carcinoma and 88 healthy controls. Results The frequency of NAT2 slow genotypes was 26.1%(18/69)in patients compared with 14.8%(13/88)in controls (P<0.05). Bladder cancer risk in patients with NAT2 slow genotypes was 2 fold as high as that in patients with NAT2 rapid genotypes. When NAT2 rapid genotypes /non-smoker were used as reference, bladder cancer risk increased to 5.8-fold (P< 0.05). Among the smokers with PY higher than 10, the patients showed a higher frequency of NAT2 slow genotype than controls (P<0.05). It was also shown that the patients with slow NAT2 genotypes were more likely to have high grade tumor(P<0.05) and advanced stage tumor (P<0.01). Conclusion The results suggest that NAT2 genetic polymorphism is associated with bladder cancer susceptibility. People with NAT2 slow genotype have higher bladder cancer risk.