摘要
研究了猪胰脂肪酶的仿生膜、吸附 -絮凝组合固定化新工艺 ,考察了该组合固定化脂肪酶体系用于橄榄油水解的性能。最终试验结果表明 :该组合固定化脂肪酶水解反应体系的反应转化率达到 5 4 5 % ,固定化酶质量回收率 86 7% ,酶活回收率 6 6 7% 。
A new method of immobilizing PPL by bio-mimic membrane with absorption-flocculation was studied. Moreover, the elementary characters of this immobilization system were demonstrated and analyzed. The experiments showed that the reaction conversion yield of hydrolyzing olive oil by this immobilized PPL enzyme is 54 5%, enzyme-quality recovery is 86 7%, and enzyme-activity recovery is 66 7%, which are all superior to the immobilized enzyme with single absorption or flocculation method.
出处
《化工进展》
EI
CAS
CSCD
北大核心
2004年第6期645-649,共5页
Chemical Industry and Engineering Progress
基金
国家自然科学基金 (No .2 99760 19
No .2 0 1760 18
No .2 0 3 760 3 4)
江苏省自然科学基金 (No .BK2 0 0 3 0 84
No .BS2 0 0 3 0 3 0 )资助项目
