高糖刺激肾小球系膜细胞葡萄糖转运蛋白4及p21的表达及其意义

Expression of glucose transporter 4 and cyclin kinase inhibitor p21 in cultured glomerular mesangial cells stimulated by high glucose

目的探讨早期糖尿病肾病(DN)肾小球系膜细胞(GMC)中葡萄糖转运蛋白(GLUT)4、p21mRNA表达变化及其与GMC肥大的关系。方法大鼠1097系膜细胞株分为高糖组、甘露醇组、不同浓度胰岛素组、高糖加不同浓度胰岛素组、正常对照组。用RT-PCR法检测各组GLUT4mRNA、p21mRNA的表达。流式细胞仪测各组GMC体积大小。结果正常对照组GMC有一定GLUT4mRNA、p21mRNA表达。高糖组GLUT4mRNA表达明显下降,p21mRNA表达明显增加。胰岛素刺激GMCGLUT4mRNA表达存在浓度依赖关系。p21mRNA表达越高,细胞前向角度散射光(FSC)越强,GMC体积越大。结论高糖刺激导致GMC肥大,GMC的p21mRNA表达上调和GLUT4mRNA表达下调与DN早期GMC肥大-肾小球肥大有关。

Objective To elucidate the relationship between expressive level alteration of glucose transporter 4(GLUT 4) mRNA,cyclin kinase inhibitor p21 mRNA and glomerular mesangial cell(GMC) hypertrophy in cultured rat GMC.Methods Cultured rat 1097 GMCs were divided into high glucose group, mannitol group, different insulin concentration groups, high glucose plus different insulin concentration groups and control group. Semi quantity RT PCR and flow cytometery were used to detect GLUT 4mRNA and p21mRNA levels and GMC volume. Results A certain expression of GLUT 4mRNA and p21mRNA from GMC was found in control group. High glucose decreased GLUT 4mRNA level and increased p21mRNA level. Insulin up regulated GLUT 4mRNA expression in a dose dependent manner.The more p21mRNA expressed, the stronger forward scatter (FSC) was and the bigger GMC became. Conclusions High glucose can cause GMC hypertrophy. Up regulation of p21mRNA and down regulation of GLUT 4mRNA may be involved in GMC hypertrophy and glomerular hypertrophy in early diabetic nephropathy.