摘要
Objectives: To compare multiplex fluorescent PCRwith serum type-specific antibody detection in thediagnosis of herpes simplex virus (HSV) infection andto evaluate its significance in the diagnosis of genitalherpes.Methods: We detected HSV infection in 121 speci-mens collected from patients with genital herpesusing both multiplex fluorescent PCR and serum type-specific antibody detection. HSV viral isolation wasused as the standard control.Results: When compared with the viral isolation, thesensitivity and specificity for multiplex fluorescentPCR were 100% and 88.89%, respectively afterdiscrepant analysis. The sensitivity and specificity fortype-specific antibody detection was 77.68 % and77.78 %, respectively. However, the type-specificantibody detected HSV in two asymptomatic patientswhile the multiplex fluorescent PCR couldn’t detectany HSV DNA from those specimens.Conclusions: Multiplex fluorescent PCR is a verysensitive and specific method for detection and typingof HSV in the lesion of genital herpes, it failed todetect HSV DNA from the asymptomatic patients.Serum type-specific antibody detection was a lesssensitive and specific test but could detect the specificantibody from some asymptomatic patients. Thecombination of these two techniques would allow rapid,sensitive and accurate detection and typing of HSVand help clinical diagnosis and epidemiologic survey-ing of genital herpes.
目的比较荧光多重PCR和血清型特异性抗体测定在生殖器疱疹临床诊断中的应用价值及评价各自的优缺点。方法以细胞培养法作为"金标准"对照,分别用荧光多重 PCR 和血清型特异性抗体检测法对121 例临床诊断为生殖器疱疹的标本进行检测。结果以培养法作标准,并通过结果的差异性分析,荧光多重PCR 的敏感性为100%,特异性为88.89%;血清型特异性抗体测定则分别为77.68% 和77.78%,荧光多重 PCR 的敏感性和特异性均显著高于血清型特异性抗体测(P<0.05),但前者不能检测出无皮损患者的HSV DNA,而后者可检测出无皮损患者中的HSV 抗体。结论荧光多重PCR 和血清型特异性抗体检测各有其自身的优缺点,单独用PCR和其它病毒分离的方法或单独使用血清特异性抗体检测的方法来诊断生殖器疱疹都是不完整的,均可造成漏诊。临床上将两种方法有机的结合起来应用能发挥各自的优势,取长补短,对早期、准确、快速地诊断生殖器疱疹及进行流行病学调查将有十分重要的意义和使用价值。
