摘要
Objectives: In order to investigate the role of mono-cytes and human leukocyte antigen (HLA) class II an-tigen in herpes simplex virus-2 (HSV-2) infection, westudied the effect of HSV-2 infection in vitro on theexpression of HLA class II antigen on monocytes.Methods: Monocytes were infected with HSV-2(Strain 333). Culture cells were collected 1, 3, 5 and 7days after infection. The levels of expression of HLAclass II antigen were measured by using alkaline phos-phatase antialkaline phosphatase method (APAAP).Results: The levels of the expression of HLA class IIantigen on monocytes significantly decreased on thefirst day of post-infection, and then gradually returnedto levels found in the controls by the 7th day post-infection.Conclusion: HLA class II antigen expression onmonocytes was inhibited with HSV-2 infection, whichmight be one means of virus escape at an early phase.The expression of HLA class II antigen may play animportant role in herpes simplex viurs-2 pathogenic-ity and immunity.
目的研究单纯疱疹病毒2 型(HSV-2)对人单核细胞表达HLA-II 类抗原(HLA-DR、HLA-DQ)的影响,探讨单核细胞及HLA-II类抗原在单纯疱疹病毒2型感染中的作用。方法用单纯疱疹病毒2型(333株)感染人单核细胞,于感染后1、3、5、7 天分别收集细胞,用APAAP 法检测单核细胞HLA-DR、HLA-DQ表达水平。结果单纯疱疹病毒2 型感染后1 天,单核细胞表达HLA-KR、HLA-DQ 抗原水平明显降低,以后逐渐升高,至7 天达到对照组水平。结论单纯疱疹病毒2 型感染早期能抑制单核细胞表达HLA-II类抗原,HSV-2抑制单核细胞表达HLA-II类抗原可能是HSV-2入侵机体的手段之一。HLA-II类抗原在单纯疱疹病毒2型致病及免疫过程中可能起重要作用。
