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金黄色葡萄球菌致病因子检测的PCR方法 被引量：12

Detection of staphylococcus aureus pathogenetic factor by PCR
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摘要 介绍了金黄色葡萄球菌致病因子检测的聚合酶链式反应 (PCR)方法 ,已取得的成果和存在的问题 ,这些方法也同样适用于其它致病菌的检测。 PCR had been described emphatically for the detection of staphylococcus aureus pathogenetic factor,especially for its development and some problems in use.These methods can be used for detection of other bacterial pathogens.
作者 王小红 谢笔钧 史贤明
机构地区 华中农业大学食品学院
出处 《食品与机械》 CSCD 2004年第3期48-50,共3页 Food and Machinery
关键词 金黄色葡萄球菌 致病因子 检测方法 PCR方法 聚合酶链式反应 毒素 Staphylococcus aureus Pathogenetic factor PCR Detection
分类号 R115 [医药卫生—公共卫生与预防医学]
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参考文献18

  • 1Martin M Dinges, Paul M Orwin, and Patrick M. Schlievert Exotoxins of Staphylococcus ameus[ J ]. Clinical microbiology reviews,2000,13(1) : 16 - 34.
  • 2Walter E, Hill, Atin R. Datta et al. Chapter 24. Identification of Foodbome bacterial pathogens by gene probes[ M]. Bacteriological Analytical Manual Online(FDA-CFSAN), 2001,1.
  • 3Johnson WM,Tyler SD, Ewan Ep et al. Detection of genes for enterotoxins,exfoliative toxios,and toxic shock syndrome toxin 1 in Staphylococcus aureus by the polymerase chain reaction[J] .J Clin Microbiol , 1991,29(3) :426 - 430.
  • 4Karsten Becker, Ricarda Roth, and Georg Peters Rapid and Specific Detection of Toxigenic Staphylococcus aureus: Use of Two Multiplex PCR Enzyme Immunoassays for Amplification and Hybridization of Staphylococcal Enterotoxin Genes, Exfoliative Toxin Genes, and Toxic Shock Syndrome Toxin 1 Gene[J]. Journal of Clinical Microbiology, 1998, 36(9) :2548 - 2553.
  • 5Karsten Becker, Ricarda Roth, Georg Peters. Rapid and Specific Detection of Toxigenic Staphylococcus aureus : Use of Two Multiplex PCR Enzyme Immunoassays for Amplification and Hybridization of Staphylococcal Enterotoxin Genes, Exfoliative Toxin Genes, and Toxic Shock Syndrome Toxin 1 Gene[J]. Journal of Clinical Microbiology, 1998,36(9):2548 - 2553.
  • 6Steven R Monday . Gregory A. Use of Multiplex PCR To Detect Classical and Newly Described Pyrogenic Toxin Genes in Staphylococcal Isolates[ J ]. Journal of Clinical Microbiology, 1999, 37(10) : 3411 - 3414.
  • 7Manisha Mehrotra, Gehua Wang, Wendy M. Johnson Multiplex PCR for Detection of Genes for Staphylococcus aureus Enterotoxins, Exfoliative Toxins, Toxic Shock Syndrome Toxin 1, and Methicillin Resistance[J].Journal of Clinical Microbiology, 2000,38 (3) : 1032 - 1035.
  • 8Wilson IG, Cooper JE, Gihnour A. Detection of enterotoxigenic Staphylococcus aureus in dried skimmed milk: use of the polymerase chain reaction for amplification and detection of staphylococcal enterotoxin genes entB and entC1 and the thermonuclease gene nuc[J]. Appl Environ Microbiol, 1991,57(6) : 1793 - 1798.
  • 9Tsen HY, Chen TR. Use of the polymerase chain reaction for specific detection of type A, D and E enterotoxigenic Staphylococcus aureus in foods[ J ]. Appl Microbiol Biotechnol, 1992,37 (5) : 685 - 690.
  • 10Naresh K Sharma, Catherine E D Rees et al. DaM Development of a Single-Reaction Multiplex PCR Toxin Typing Assay for Staphylococcus Strains[J]. Applied and Environmental Micrabiology, 2000,66(4): 1347 - 1353.

二级参考文献8

  • 1Reginald W, Bennett. Chapter 13A. Staphylococcal enterotoxins: Micro-slide double diffusion and ELISA-based methods. Bacteriological Analytical Manual Online (FDA-CFSAN), 2001, 1
  • 2中国国家标准：GB4789．17—24—94食品卫生微生物学检验(肉、乳、蛋制品，水产、饮料、调味品、糖果、糕点、果脯)
  • 3中华人民共和国国家标准：GB 4789．10-94食品卫生微生物学检验．金黄色葡萄球菌检验
  • 4Reginald W. Bennett and Gayle A, Lancette. Chapter 12. Staphylococcus aureus. Bacteriological Analytical Manual Online (FDA-CFSAN), 2001.1.
  • 5Peter Feng. Appendix 1. Ripid methods for detecting food borne pathogens. Bacteriological Analytical Manual Online (FDA-CFSAN), 2001. 1
  • 6Avranham Rasooly. Chapter 13B. Eleetrophoretic and Immunoblot Analysis of Staphylococcal Enterotoxirrs in Food. Bacteriological Analytical Manual Online (FDA-CFSAN), 2001, 1
  • 7Walter E, Hill, Atin R. Datta et al. Chapter 24. Identification of Foodbome bacterial pathogens by gene probes. Bacteriological Analytical Manual Online (FDA-CFSAN), 2001, 1
  • 8杨朝国,王明谊,吴磊.金黄色葡萄球菌DNA扩增分型的应用[J].国外医学（临床生物化学与检验学分册）,1999,20(6):267-269. 被引量：3

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食品与机械
2004年 第3期

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