摘要
目的 了解放射损伤条件下骨髓细胞 (包括基质细胞和造血细胞 )生物力学特性的改变。方法 以 6Gy6 0 Coγ射线照射昆明系小鼠制备动物模型 ,以细胞电泳法检测骨髓细胞的细胞电泳率 ,荧光探针法测定细胞膜微黏度 ,旋转法检测细胞的黏附性 ,核孔滤膜法检测细胞的变形指数。结果 放射损伤后骨髓细胞的变形能力降低 ,细胞膜微黏度增加 ,细胞的黏附率和电泳率显著降低。结论 6Gy6 0 Coγ射线对骨髓细胞的生物力学特性有显著影响 。
Objective To explore the change of bone marrow cell biomechanical characteristics in radiation-injured mice and the influencing factors. Methods Male Kumming mice were exposed to total body irradiation of 6 Gy γ-rays from a 60 Co source.Electrophoresis,DPH probe,micropore filter,and adhesion rate methods were used to detect cell surface charge,membrane microviscosity,cell deformability,and cell adhesion,respectively. Results The deformability,adhesiveness and cell surface charges of bone marrow cells(including hematopoietic cells and stromal cells)were dramatically decreased,but membrane microviscosity was obviously increased after irradiation on 1 d,3 d and 7 d. Conclusion The biomechanical characteristics of bone marrow cells are obviously changed after radiation injury.It might be one of the reasons of hematopoietic failure after irradiation.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2004年第3期231-233,共3页
Chinese Journal of Radiological Medicine and Protection
基金
全军"九五"攻关课题基金资助项目
关键词
6Cyγ射线
骨髓细胞
生物力学
黏附性
表面电荷
变形性
Biomechanical characteristics
Bone marrow cells
Adhesiveness
Surface charge
Deformability
