尖吻蝮蛇蛇毒对刚地弓形虫侵入宿主细胞的作用研究

Influence of snake venom of Agkistrodom acutus on the invasion of Toxoplasma gondii tachyzoites to host cells

目的 观察细胞培养系统中宿主细胞在不同剂量源自皖南地区的尖吻蝮蛇 (Agkistrodonacutus)蛇毒的作用下感染刚地弓形虫速殖子百分率的差异 ;探讨运用蝮蛇蛇毒作为辅助因子提高细胞培养用于弓形虫病病原学诊断的敏感性。方法 以尖吻蝮蛇蛇毒加入分孔培养的Hela细胞和THP - 1细胞中 ,分别用IFA和流式细胞仪进行检测 ,观察尖吻蝮蛇蛇毒对弓形虫速殖子侵入细胞的影响。结果 在相同的时间内 ,在尖吻蝮蛇蛇毒的作用下 ,Hela细胞和THP - 1细胞感染弓形虫速殖子的百分率在不同的时段均明显的提高。结论 尖吻蝮蛇蛇毒可以促进弓形虫速殖子侵入宿主细胞 ,在实际应中 ,可以作为一种辅助因子在检测标本时提高速殖子检出率 。

The aim of this study is to investigate the influence of the snake venom of Agkistrodom acutus from the southern area of Anhui province on the invasion of Toxoplasma gondii tachyzoites to host cells in order to explore the possibility to use this kind of snake venom for the diagnostic means for toxoplasmosis The snake venom was added to the Hela cell and THP 1cell cultures cultivated with tachyzoiyes, and then in the different times of cultivation, the infected cells were counted and examined by IFA and FCM. It was found that the rate of Hela cells and THP 1 cells infected by tachyzoites was significantly increased in comparison with those of the controls.It concludes that the snake venom of A acutus can promote the tachyzoites to invade host cells in a given dose, so it is possible to use this snake venom as an adjuvant to increase the positive rate and to reduce the time for detection of tachyzoites.