鳗弧菌pEIB1质粒缺失株MVAV6201的筛选和三价铁源供给的优化
被引量：2
摘要
以鳗弧菌Vibrio anguiUarum MVM425(01型)为出发菌株，通过变温培养诱变筛选得到pEIB1质粒缺失株MVAV6201。通过与野生株MVM425的摇瓶培养对比发现，Fe^3+营养的摄取是质粒缺失株MVAV6201生长的瓶颈。MVAV6201对Fe^3+的吸收有赖于外界Fe^3+营养的供给形式。柠檬酸铁铵能有效增强MVAV6201对三价铁营养的吸收，促进菌体细胞的生长。柠檬酸铁铵饱和浓度为0．4mmol／L。
出处
《高技术通讯》
EI
CAS
CSCD
2004年第5期64-67,共4页
Chinese High Technology Letters
参考文献17
-
1[1]Bullen J J, Griffiths E. Iron bingding proteins and host defense. In Bullen JJ, Griffiths E (ed.). Iron and Infection. 2nd ed. London: John Wiely & Sons, 1999, 327.
-
2[2]Crosa J H. Microbiol Mol Biol Rev, 1997, 67: 319
-
3[3]Crosa J H. Molecular genetics of iron transport as a component of bacteria virulence, In: Bullen JJ, Griffiths E (ed.). Iron and Infection. 2nd. London: John Wiely & Sons, 1999, 225
-
4[4]Crosa J H, Schiewe M H, Falkow S. Infect Immun, 1977, 27:509
-
5[5]Martinez J I, Herrero M, de Lorenzo V. J Mol Biol, 1994, 238: 288
-
6[6]Neilands J B. J Microbiol, 1992, 38: 728
-
7[7]Harbell S O, Hodgins H O, Schiewe M H. J Fish Dis, 1979, 2: 527
-
8[8]Crosa J H. Nature, 1980, 284: 566
-
9[9]Pedersen K, Gram L, Austin D A, et al. J Appl Microbiol, 1997, 83: 365
-
10[10]Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: a Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, 1989, 108
同被引文献21
-
1萨姆布鲁克J,弗里奇EF.分子克隆手册[M].第2版.北京:科学出版社,1998:16-87.
-
2Zhou Y C,Su Y Q.Ultrasonic immunization of sea bream,Pagrus major (Temminck & Schlegel),with a mixed vaccine against Vibrio alginolyticus and V.anguillarum[J].J.Fish Dis,2002,25 (6):325-331.
-
3Norqvist A,Hagstr M A.Protection of rainbow trout against vibriosis and furunculosis by the use of attenuated strains of Vibrio anguillarum[J].Appl.Environ.Microbiol,1989,55 (6):1400-1405.
-
4Fang H M,Ling K C,Ge R,et al.Enhancement of protective immunity in blue gourami,Trichogaster trichopterus (Pallas),against Aeromonas hydrophila and Vibrio anguillarum by A.hydrophila major adhesin[J].J.Fish Dis,2000,23 (2):137-145.
-
5Xia Y J,Wen W H,Huang W O,et al.Development of a phage displayed disulfide-stabilized Fy fragment vaccine against Vibrio anguillarum[J].Vaccine,2005,23 (24):3174-3180.
-
6Crosa J H.Signal transduction and transcriptional and posttranscriptional control of iron-regulated genes in bacteria[J].Microbiol Mol.Rev.,1997,61 (3):319-336.
-
7Harbell S O,Hodgins H O,Schiewe M H,et al.Studies on the pathology of vibriosis in coho salmon[J].J.Fish Dis,1979,2 (5):527-535.
-
8Crosa J H.A plasmid associated with virulence in the marine fish pathogen Vibrio anguillarum specifies and iron-sequestering system[J].Nature,1980,284(5756):566-568.
-
9Pedersen K,Gram L,Austin D A,et al.Pathogenicity of Vibrio anguillarum serogroup O1 strains compared to plasmids,outer membrane protein profiles and siderophore production[J].J.Appl.Microbiol,1997,82 (3):365-371.
-
10Chen Q,Crosa J H.Chromosome-mediated 2,3-dihydroxybenzoic acid is a precursor in the biosynthesis of the plasmid-mediated siderophore anguibactin in Vibrio anguillarum[J].J.Bacter,1994,176 (14):4226-4234.
-
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-
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-
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-
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-
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-
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-
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-
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-
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