精子运动能力与一氧化氮关系的探讨

Relationship between sperm movement and nitric oxide in sperm

目的 探讨一氧化氮 (NO)与精子运动能力的关系。方法 采用镀铜镉还原荧光法 ,测定人精液中NO代谢产物硝酸盐 (NO3 -)。参照WHO方法 ,在超高倍显微镜下观察精子存活率、活动力等。结果 80例男性不育者其中 15例NO浓度明显低于正常对照组精子活动力a +b级 <5 0 % (P <0 .0 1) ,以运动轨迹异常和头摆动幅度下降为主 ,精子存活力 >75 %无明显差异 (P >0 .0 5 ) ;6 5例NO浓度显著高于正常对照精子活动力a +b级 <5 0 % ,存活率 <75 % (P <0 .0 0 1)。结论 NO与精子运动功能有着密切关系。高浓度时明显抑制精子活动力及存活率。低浓度时精子存活率及功能有着维护作用 ,这对男性不育的病因研究和治疗有非常重要的临床价值。

Objective: To study the relationship between Sperm movement and nitric oxide in sperm. Methods: Fluorescence reducibility by copper-coated cadmium was used to detect nitrate from metabolism of nitric oxide in sperm. Results: In 80 sperm samples,there were 65 samples that concentration of nitric oxide is higher than hardly that in control group which activity grade a+b is <50% and livability is <75% ( P <0.001). Conclusion: There was significant relationship between sterility and nitric oxide in sperm.