核因子κB在妊高征患者胎盘组织的定位及定量研究

The role of nuclear factor-κB in the pathogenesis of PIH

目的 探讨核因子κB(NF -κB)在妊高征患者胎盘血管平滑肌及内皮细胞中表达的变化及其在妊高征发病过程中的作用。方法 HE染色后镜下观察胎盘组织及血管的病理变化 ,免疫组织化学方法及Westernblot检测妊高征患者胎盘组织I-κB、NF -κB的表达。结果 HE染色显示妊高征胎盘绒毛血管变细、数目减少、血管合体膜增厚、胎盘细小血管平滑肌细胞增生、纤维素样坏死明显多于正常妊娠 ;免疫组织化学显示妊高征患者胎盘血管平滑肌细胞及内皮细胞NF -κB胞浆、胞核染色较正常妊娠明显增强 ;Westernblot显示妊高征患者胎盘组织胞浆、胞核NF -κB含量、胞核 /总NF -κB含量比值明显高于正常妊娠 ;妊高征患者胎盘组织胞浆I-κB含量明显低于正常妊娠。结论 NF -κB的激活及增加可能为妊高征发病机制的重要信息途径之一。

Objective: To study the changes of nuclear factor-κB(NF-κB) activity in the placenta of normotensive and pregnancy induced hypertention(PIH). Methods: The NF-κB expression and activity in placental tissue were determined by immunohistochemistry, image alalysis and western blot. Resouts: The thinkening, fibrinoid necrosis and other pathological changes of the placeta vessel in PIH were signficantly higher than those in normal group. The expression of NF-κB in placental blood vessel of PIH was greatly stronger than that in normal group. The amount of total, cytosoic and nuclei NF-κB and the ratio of cytosoic to total NF-κB amount in PIH were higher than those in normal group. The amount of total cytosoic I-κB in PIH were lower than those in normal group. Conclusion: The NF-κB signal pathway may regulates the expression of placenta and arterial collagen in PIH, which may play an important role in the pathogenesis of PIH.