3,3’-二氯联苯胺以不同染毒方式对A系小鼠致瘤作用的研究

Study on the blastomogenic action of DCB upon strain A mice by different administration

目的 :探讨3 ,3’ -二氯联苯胺 (DCB)对A系小鼠的致瘤作用。方法 :采用两种不同染毒方式。其一是吸入式染毒 ,即利用动式吸入染毒装置 ,使A系小鼠吸入不同剂量DCB(5,15 ,45mg/kg) ,每周两次 ,染毒4个月后剖杀全部小鼠 ,检查肺腺瘤的发生情况 ;另一种是经口染毒 ,即经口喂饲A系小鼠不同剂量DCB(5.5,21.9,65.8,136.2mg/kg)5个月 ,停药1个月后剖杀全部小鼠 ,重点检查肺、肝、肾的肿瘤发生情况 ,并对肉眼可见的肿瘤进行病理学检查。结果 :在吸入式染毒试验中 ,各剂量组肺腺瘤发生率与对照组相比 ,差别均无统计学意义 (P>0.05) ;在经口染毒试验中 ,A系小鼠肝脏和肺脏均有肿瘤发生。最高剂量组的总肿瘤发生率和恶性肿瘤发生率 (51.1%和37.8% )与对照组 (17.6 %和0)相比 ,差别有统计学意义 (P<0.01,P<0.05)。结论 :在本实验条件下 ,经口喂饲DCB可诱发A系小鼠发生恶性肿瘤。

Objective: To study the blastomogenic action of 3,3'-dichlorobenzidine (DCB) upon strain A mice.Methods: Strain A mice were administered DCB by two different ways. One way is inhalation. Strain A mice in 3 groups inhaled DCB in 3 different dosages (5, 15, 45 mg/kg) respectively, twice a week. After 3 months, all the mice were killed and their lungs were examined anatomically for lung adenomas. The other way is oral administration. Strain A mice in 4 groups were orally administered DCB in 4 different dosages (5.5, 21.9, 65.8, 136.2 mg/kg) respectively for 5 months. One month after stopping DCB, all the mice were killed and the viscera, especially the lungs, the livers and the kidneys were examined anatomically, and the tumors found in these organs were further examined pathologically.Results: In the test of inhalation, there was no significant difference between the incidence of lung adenoma of each dosage group and that of the control group(P>0.05).In the test of oral administration, benign and malignant tumors were found in both the lungs and the livers of strain A mice. The incidence of total tumors (51.1%) and malignant tumors (37.8%) of the highest dosage group were higher than those of the control group (17.6%, 0) (P<0.01,P<0.05).Conclusion: Under the experimental condition, DCB by oral administration can induce malignant tumors in strain A mice.