摘要
目的 :检测原发性肝癌患者外周血IL -2,sIL -2R,TNFa水平及NK细胞活性 ,并探讨患者细胞免疫功能低下的原因。方法 :采用MTT法检测30例原发性肝癌患者外周血单个核细胞白细胞介素2(IL -2)的诱生水平 ;采用ELISA法检测血清可溶性白细胞介素2受体 (sIL -2R) ,肿瘤坏死因子a(TNFa)水平 ;自然杀伤细胞活性 (NK -A)检测采用MTT法。结果 :原发性肝癌患者外周血TNFa水平降低 (P<0.01) ;IL -2、NK -A活性水平低于对照组 (P<0.05) ;sIL -2R水平高于对照组 (P<0.01)。结论 :原发性肝癌患者Th1细胞因子及NK -A活性有所降低 ,检测该指标有助于了解患者机体细胞免疫功能及预后评估。
Objective: To investigate the levels of IL-2,sIL-2R,TNFa and NK cell activity and the causes of insufficient cellular immunity in patients with primary hepatic carcinoma. Methods:The levels of IL-2,NK activity in PBMC were measured in 30 patients with primary hepatic carcinoma by MTT; The levels of sIL-2R,TNFa in serum were measured by an enzymelinked immunosorbent assay(ELISA).Results:The levels of TNFa and IL-2,NK activity were significantly lower in primary hepatic carcinoma than control group (first assayed P<0.01,secend assayed P<0.05), but sIL-2R were significantly higher than in control group(P<0.01).Conclusion:The cytokines and NK activity are decrease in patients with primary hepatic carcinoma. It is probably the causes for insufficient cellular immunity.
出处
《天津医科大学学报》
2004年第2期280-281,287,共3页
Journal of Tianjin Medical University
