p53基因在病理性瘢痕成纤维细胞中的表达

p53 gene expression in fibroblast of pathological keloid

目的 探讨 p5 3基因在病理性瘢痕成纤维细胞中的表达情况及其意义。方法 取手术切除的正常皮肤、增生性瘢痕和瘢痕疙瘩组织各 8例为标本 ,通过细胞培养 6～ 8代后 ,应用反转录 -聚合酶链反应 (RT-PCR)的方法检测 p5 3m RNA的表达。结果 p5 3m RNA在正常皮肤高表达 ,而在增生性瘢痕和瘢痕疙瘩组明显减弱 ,差异具有显著性 (P<0 .0 5 )。结论 p5 3基因介导了瘢痕疙瘩成纤维细胞的异常增生 ,是病理性瘢痕发生的分子机制之一。

Objective To study p53 gene expression of fibroblasts isolated from different tissues.Methods The fibroblasts from 8 cases of normal skin,8 hypertrophic scars and 8 keloids were cultured respectively in vitro.RT-PCR technique was performed to detect the expression of p53 mRNA.Results p53 mRNA expression on hypertrophic and keloid scars was significantly lower than normal skin controls (P<0.05).Conclusion p53 gene may contribute to abnormal proliferation of fibroblasts in keloid,it is one of molecular mechanisms in pathological epulosis.