心腔内注射腺病毒携带肝细胞生长因子转染大鼠急性缺血心肌的实验研究

EFFECTS OF ADENOVIRUS-MEDIATED HEPATOCYTE GROWTH FACTOR INFUSED INTO LEFT VENTRICULAR CAVITY ON ACUTE ISCHEMIC MYOCARDIUM

目的 分析研究腺病毒携带肝细胞生长因子 (Ad -GFP)心腔内注射转染对大鼠缺血心肌的影响。方法 将4 5只Wistar大鼠随机分为心室腔注射Ad -HGF组 (Ⅰ组 ) ,心室腔注射携带绿色荧光蛋白 (Ad -GFP)组 (Ⅱ组 ) ,心室腔注射生理盐水对照组 (Ⅲ组 ) ,各组分别于术后 3,7,14 ,2 1,2 8d各处死 3只进行有关指标的检测。结果 心室腔注射Ad -HGF组术后 3d心脏冷冻切片出现绿色荧光 ,14d消失 ,14 ,2 1,2 8d可见大量的新生血管生成 ,较对照组有明显的增加。结论 心室腔注射Ad -HGF组见微小血管大量生成 。

Objective To study the effects of adenovirus-mediatedhepatocyte growth factor infused into left ventricular cavity on acute ischemic myocardium. Methods 45 rats were divided into three groups randomly.A volume of 100μl normal saline containing 1×10 9 plaque forming units(pfu) of recombinant Ad-HGF,Ad-GFP or pure saline was infused into left ventricular cavity,respectively.Thetransfected Ad-HGF expressions were evaluated on days of 3,7,14,21,28 after operation. Results The fluorescent expression in group infused into left ventricular cavity appeared on 3 days and persisted up to 14 days.The amount of capillary vessels was counted on day of 3,7,14,21,28 after operation.The number of vessels was more significantly increased in group transfected Ad-HGF than that of control group. Conclusion The administration of Ad-HGF increas the number of vessels in regional myocardium.The pathway of Ad-GFP infusion into left ventricular cavity group is effective on acute ischemic myocardium.