尖锐湿疣HPV导流杂交快速基因分型及临床意义

Rapid Typing of HPV by Reverse Dot Blot Hybridization and Its Clinical Characteristics Among Patients with Condyloma Acuminata

目的 :探讨外生殖器及子宫颈尖锐湿疣患者皮损脱落细胞或组织中人乳头瘤病毒 (HumanPa pillomaVirus,HPV) 9种常见的基因亚型感染分布状况及临床意义。方法 :采集患者生殖器或子宫颈尖锐湿疣组织或脱落细胞样本 ,分离DNA ,PCR扩增后与 9种常见HPV亚型探针进行导流快速反斑点印迹杂交检测HPVDNA。结果 :经PCR扩增并通过通用探针杂交分析后 ,1 0 8例患者标本中 ,1 0 1例标本呈HPVDNA阳性 ,7例HPV阴性。HPVPCR阳性标本中 ,98例成功分型。 5种高危型HPV 1 6,1 8,3 1 ,3 3 ,5 8的检出率分别为 3 1 5 % ( 3 4/ 1 0 8)、1 6 7% ( 1 8/ 1 0 8)、6 5 % ( 7/ 1 0 8) ,4 6% ( 5 / 1 0 8) ,1 3 0 % ( 1 4/ 1 0 8)。三种低危型HPV6,1 1 ,5 3型的检出率分别为 41 7% ( 4 5 / 1 0 8) ,5 6 5 % ( 61 / 1 0 8)和 6 5 % ( 7/ 1 0 8)。危险度待定HPV亚型CP83 0 4检出率为 4 6% ( 5 / 1 0 8)。结论 :结论HPV 1 1、6、1 6型感染是尖锐湿疣的主要型别。

Objective:To study the spectrum of nine types of HPV and its clinical characteristics in specimens of dropping cells or lesions from patients with genital or cervical condyloma acuminata. Methods:DNA was isolated from specimens of dropping cells or lesions and detected by PCR amplification. 9 types of probes for different HPV genotype were used for hybridization to detect HPV DNA. Results:After PCR amplification and hybridization, 101 samples were HPV positive, others were negative. After reverse Dot Blot analysis, the detecting rates of 5 high risk HPV genotypes 16, 18, 31, 33, 58 were 31.5%(34/108), 16.7%(18/108), 6.5%(7/108), 4.6%(5/108) and 13.0%(14/108) respectively, while the detecting rates of 3 low risk HPV genotypes 6, 11, 53 were 41.7%(45/108), 56.5%(61/108) and 6.5% (7/108) respectively. For CP8304, it was 4.6%(5/108) in positive. Conclusion:HPV 6,11,16 are the main genotypes in the pathogenesis of condyloma acuminata.