甲强龙对脑缺血早期星形胶质细胞的保护作用

Methylprednisolone for protection of astrocytes during earlier period of cerebral ischemia

目的:研究甲强龙对缺血再灌脑损伤早期的保护作用及其可能机制。方法:利用大脑中动脉线栓法制作脑缺血动物模型,缺血3h,再灌3h,术后立即腹腔注射甲强龙60mg/kg,再灌3h后,观察行为学指标并评分。利用免疫组织化学方法,观察胶质原纤维酸性蛋白(glialfibrillaryacidicprotein,GFAP)及肿瘤坏死因子(tumornecrosisfactorα,TNF-α)的表达。结果:甲强龙治疗组和缺血再灌模型组比较,反应性星形胶质细胞数量减少(P<0.05),形态趋于正常,治疗组神经功能评分为(1.14±0.38)分和模型组(2.14±0.69)分比较有所改善(t=2.69,P<0.05),TNF-α的表达和模型组比较数量减少(P<0.05)。结论:早期应用甲强龙可以改善脑缺血损伤所致的功能障碍,这一作用和星形胶质细胞有关,也和早期炎症因子TNF-α的表达有关。

AIM:To investigate the protective effect and possible mechanism of methylprednisolone(MPSS) during earlier period of cerebral ischemia reperfusion injury. METHODS:The model of focal cerebral ischemia reperfusion in rats was established by blocking middle cerebral artery,then ischemia 3 hours and reperfusion 3 hours.MPSS at a dose of 60 mg/kg was injected intraperitoneally after operation,after 3 hours of reperfusion,motor functional index were observed and scored.Immunohischemical method was used to observe and quantitatively analyse the expression of glial fibrillary acidic protein(GFAP) and tumor necrosis factor α(TNF α). RESULTS:Compared with the model group,the number of reactive astrocytes in MPSS treatment group was decreased(P < 0.05) and their forms tended to normal.Compared with the model group(2.14±0.69),neural functional index in the treatment group(1.14±0.38) was improved(t=2.69,P< 0.05).Compared with the model group, the expression of TNF αwas decreased(P< 0.05) in the treatment group. CONCLUSION:MPSS used at earlier period of ischemia reperfusion injury can ameliorate the motor dysfunction, which may be related to the astrocytes and also to TNF αexpression in earlier inflammatory factors.