加减二仙汤对脑缺血大鼠右心耳内分泌功能反应性变化的量效关系研究

Dose-effect relation of reactive changes of endocrine particles of right atrial auricle in cerebral ischemic rats treated with modified erxian tang

目的:通过研究急性不完全脑缺血大鼠右心耳内分泌颗粒对加减二仙汤反应性的量效关系,探讨其变化的意义。方法:中老年Wistar大鼠,采用双侧颈总动脉夹闭2h时后去夹再通的方法,制作急性不完全脑缺血模型。实验组大鼠造模前给予加减二仙汤煎剂,于血流再灌后6,24h,3,7d处死。取大鼠右心耳制作超薄切片,电镜观察、摄片并进行形态计量法测试。结果:再灌6,24h组对照组大鼠右心耳内分泌颗粒明显减少,实验组仅少量释放,3d后颗粒由少渐多,由小变大,电子密度升高,7d后趋于正常。形态计量法测试统计结果心房特殊颗粒(atrialspecificgranules,ASG)的体密度,面密度,面数密度,平均直径实验组与对照组比较差异有显著性意义,尤其是24h组以上几项为(0.071±0.085)μm3/μm3,(7.94±1.47),(3.10±0.08)nm,(0.368±0.103)μm,与同期对照组(0.049±0.005)μm3/μm3,(7.34±2.33),(2.27±0.07)nm,(0.290±0.091)μm比较,差异有显著性意义(P<0.05)。结论:加减二仙汤明显减少了实验组大鼠右心耳心肌纤维内ASG的释放和消耗,根据心房钠尿肽的生物学效应,实验结果反证了该方药有效的脑保护作用。

AIM:To study the dose effect relation between endocrine particles of right atrial auricle in rats with acute incomplete cerebral ischemia and modified erxian tang,and to explore the significance of these changes. METHODS:The middle aged Wistar rats were used as the models with the method of bilateral common carotid occlusion for 2 hours and then release to make acute incomplete cerebral ischemia models.The rats in the experimental group were given erxian tang before modeling.The rats in all groups were killed at 6 hours,24 hours,3 days and 7days after reperfusion and the right atrial auricle were taken out and made into ultra thin section which were observed with electron microscope,by means of morphometriology. RESULTS:The atrial specific granules(ASG) of the rats in the control group decreased obviously after reperfusion 6 and 24 hours,but only a little released in those of experimental group. Three days after the ASG increased in number,the number of granules changed from small to large and their electric density from rare to dense, gradually.The ASG was similar to normal in every respect after 7 days.The morphometric study indicated that there were remarkable differences between the experimental group and control group in the volume density(Vv),neumerical density on area(Na), neumerical density(Nv) and average diameter(D)of the ASG(P< 0.05,P< 0.01), especially in the group at 24 hours[(0.071±0.085) μm3/μm3,(7.94±1.47),(3.10±0.08) nm,(0.368±0.103) μm,respectively], which had significant difference compared with those of control group[(0.049±0.005) μm3/μm3,(7.34±2.33),(2.27±0.07) nm, (0.290±0.091) μm,respectively](P< 0.05). CONCLUSION:Releasing and consuming of the ASG in myofibre of the rats right atrial auricle in the experimental group are reduced obviously by the modified erxian tang.According to the special functions of the ANP,the effect of modified erxian tang on brain protection is disproofed by these experimental results.