柴胡桃仁汤对迟发性运动障碍模型大鼠的治疗作用

Effect of chaihu taoren tang on tardive dyskinesia of rat model

目的:观察柴胡桃仁汤对迟发性运动障碍(tardivedyskinesia,TD)大鼠模型行为及血清超氧化物歧化酶(superoxidedismutase,SOD)活力、脂质过氧化代谢物丙二醛水平变化,探讨TD可能的病理生理机制。方法:将24只雄性Sprague-Dawley(SD)大鼠随机分为空白、模型、治疗四组,每组8只,分别予腹腔注射生理盐水+第5周始灌胃生理盐水、腹腔注射氟哌啶醇+第5周始灌胃生理盐水、腹腔注射氟哌啶醇+第5周始灌胃柴胡桃仁汤处理,共9周;每周末观察大鼠口部异常运动,进行评分。研究结束时取静脉血,分离血清,测定血清超SOD活力、及丙二醛水平。结果:腹腔注射氟哌啶醇使SD大鼠口部异常运动增加明显,第5周达峰值;柴胡桃仁汤可使TD模型大鼠口周异常运动评分下降,减分为12.0±9.9,与TD组(0.6+4.2)相比,差异有显著性(u=5.0,P<0.05);TD组大鼠血清SOD活力(kat/L)明显降低(5.4±0.7),丙二醛水平(kat/L)显著升高(20.4±6.0),柴胡桃仁汤可缓解这一改变(SOD:6.5±0.3,丙二醛:7.7±3.2),而且差异均有显著性(P<0.01)。可用于制作TD动物模型;而可使这些行为异常显著缓解(P<0.05);与模型组比较,治疗组血清SOD活力升高,丙二醛水平下降,差异有显著性意义(P<0.01)。结论:柴胡桃仁汤通过阻制氧化损伤,有效缓解TD模型大鼠口周异常运动,氧化应激在TD?

AIM:To observe the effect of chaihu taoren tang(CTT) on rats model with tardive dyskinesia(TD) and the level changes of superoxide dismutase(SOD) activity,and malondialdehydescid(MDA) level in serum,and to explore the possible pathophysiological mechanism of TD. METHODS:Twenty four male Sprague Dawley(SD)rats were randomized into the control,model and treated group,8 in each group.They were processed with intraperitoneal injection of saline+saline into stomach since the fifth week,intraperitoneal injection of haloperidol+saline into stomach since the fifth week,intraperitoneal injection of haloperidol+chaihu taoren tang respectively.The duration of treatment was 9 weeks.Orofacial dyskinesia was evaluated at every weekend.Venous blood was collected at the end of research and the serum was isolated.SOD activity and MDA level in serum were assayed. RESULTS:Haloperidol could facilitate orofacial dyskinesia movement of rats to reach the peak value at the fifth week;decrease the score of orofacial dyskinesia of TD rats model to 12.0±9.9,as compared with that of TD group(0.6±4.2),showing significant difference(u=5.0,P< 0.05);Serum SOD activity(kat/L) in TD rats was obviously decreased (5.4±0.7),while MDA level(μmol/L) was increased significantly(20.4±6.0),and chaihu taoren tang could ameliorate this change(SOD:6.5±0.3,MDA:7.7±3.2),and it indicated significant difference(P< 0.01).It could used to establish TD animal model, and could ameliorate the abnormal activity(P< 0.05).Compared with the model group, serum SOD activity increased,MDA level decreased,which indicated significant difference(P< 0.01). CONCLUSION:Chaihu taoren tang can relieve orofacial dyskinesia of TD model rats effectively by preventing oxidation damage;and oxidative stress plays an important role in TD occurrence process.