羧乙基锗倍半氧化物对神经节烟碱传递的影响(英文)

Effects of carboxyethygermanium sesquioxide(Ge-132) on nicotinic transmission in rats ganglia

背景:羧乙基锗倍半氧化物(carboxyethygermaniumsesquioxide,Ge-132)是近30年研究较多的具有多种生物活性的有机锗化合物,临床试验性用于老年痴呆症和及其他多种疾病的预防和治疗。但有机锗对神经系统作用环节及机制尚未见文献报道较少。目的:探索Ge-132对大鼠颈上神经节细胞及烟碱传递过程的影响。设计:非随机自身对照实验性研究。地点和材料:实验在广西医科大学实验中心神经药理学实验室完成。材料:成年Wistar大鼠30只,雌雄兼用。干预:大鼠急性处死后,迅速将颈上神经节连同节前神经离体,用950mL/LO2和50mL/LCO2混合气饱和的,pH为7.4±0.05的克氏溶液,恒温(35±0.5)℃持续灌流。用内充灌3M-KCl溶液的玻璃微电极穿刺离体颈上神经节神经元进行细胞内记录。所有的药物均用Kreb液中配成相应浓度直接灌流神经节。主要观察指标:快兴奋性突触后电位(FEPSP)。结果:①Ge-132在1×10-4mol/L或更高浓度,可逆地抑制刺激节前神经引起的FEPSP的幅度,并可使动作电位发放频率减少。②对乙酰胆碱(ACh)引起的膜除极反应无显著的影响。③对细胞膜被动膜性质无明显的影响。结论:Ge-132对烟碱传递的抑制作用可能是其使突触前ACh释放减少所至。

BACKGROUND:Carboxyethygermanium sesquioxide(Ge 132) is an organic germanium compound with multiple bio activities which has been studied extensively in recent 30 years.It has been used as investigation of new drugs to treat and prevent Alzheimer's disease and many other diseases.But the action sites and the mechanism on nervous system of Ge 132 has yield to be established. OBJECTIVE:To study the effects of Ge 132 on neurons and on nicotinic transmission of the isolated superior cervical ganglia(SCG) of the rats. DESIGN:Non random and self control experimental study. SETTING and MATERIALS:Setting: The Lab of Neuropharmacology,The Experimental Center of Guangxi Medical University.Materials:Thirty adult Wistar rats of either sex were used. INTERVENTION:After the animals were sacrificed acutely, the superior cervical ganglia(SCG)together with their preganglionic nerve trunks were isolated rapidly.The ganglia were perfused continuously with a Krebs solution[saturated with 950 mL/L O2 and 50 mL/L CO2 ,pH 7.4 ±0.05,(35±0.5) ℃].Intracellular recording was obtained from neurons of the isolated superior cervical ganglia by means of fiber containing glass microelectrode filled with 3M KCl.All the drugs were solved in Kreb's solution,and applied to ganglia by perfusion in known concentration. MAIN OUTCOME MEASURES:Fast excitatory postsynaptic potential(FEPSP). RESULTS:①At the concentrations of 1×10-4 mol/L or more,Ge 132 reversibly depressed the amplitude and duration of FEPSP induced by preganglionic nerve stimulation and the discharge frequency of cell action potentials;②Ge 132 did not significantly change the membrane depolarization induced by application of exogenous acetylcholine(ACh);③The passive properties of the sympathetic neurons were not significantly altered by Ge 132. CONCLUSION:Ge 132 induced depression on nicotinic transmission may be explained by an action of Ge 132 on the reducing presynaptic ACh release.