摘要
目的 探讨脊髓源性神经干细胞的培养方法和体外分化特性。 方法 从孕龄 15天的胚胎大鼠脊髓组织中分离获得神经干细胞 ,采用含表皮生长因子及碱性成纤维细胞生长因子的无血清限定性培养基培养并进行干细胞体外分化 ,细胞免疫荧光化学方法鉴定分化结果。 结果 胚胎脊髓中可分离得到大量的神经干细胞 ,培养 10天左右可获得干细胞球 ;用细胞免疫荧光化学法鉴定干细胞分化 ,可见其中含有神经元和星形胶质细胞。 结论 无血清限定性培养基是脊髓源性神经干细胞的良好培养基 ;
Objective To investigate the method of cultivation and the feature of differentiation of spinal cord derived stem cells in vitro. Methods The neural stem cells from spinal cord of 15 days fetal rats were harvested and cultivated in a serum free limited medium. The stem cells were induced to differentiate and the results were identified by cellular immunohistochemistry. Results Lots of stem cells were obtained from the spinal cord of fetal rats and the sphere of stem cells was formed about 10 days. Neural stem cells can give rise to mature neurons and astrocytes. Conclusion Epidermal growth factor/basic fibroblast growth factor serum free limited medium can promote the proliferation activity of the stem cells. Spinal cord derived stem cells can differentiate into glial cells and neurons.
出处
《中国修复重建外科杂志》
CAS
CSCD
2004年第4期247-249,共3页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金资助项目 (30 2 71 30 8)~~
