期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

载脂蛋白(a)五核苷酸重复序列基因多态性与冠心病及血浆Lp(a)水平的关系 被引量：1

Association of apo(a)Pentanucleotide Polymorphism with Coronary Heart Disease and Serum Lp(a)levels
下载PDF
导出
摘要 目的 :探讨载脂蛋白[apo(a)]五核苷酸重复序列基因多态性在冠心病患者中的分布状况及其与血浆Lp(a)水平的关系。方法 :对293例经冠脉造影确诊的冠心病患者及163例正常对照组进行apo(a)基因多态性频率分析及血浆Lp(a)水平的检测。PCR法扩增相应的apo(a)DNA片段 ,聚丙烯酰胺凝胶电泳分离扩增产物并确定其基因型。ELISA法测定血浆Lp(a)水平。结果 :共检出8种等位基因 ,19种基因型 ,常见的基因型为8/8、8/9、5/8、9/94种。2组中5/8基因型携带者的血浆Lp(a)水平均高于其他基因型携带者 (P<0.05) ;高Lp(a)组5/8基因型携带者多于低Lp(a)组 (P<0.05) ;且高Lp(a)组中冠心病患者所占比例明显高于低Lp(a)组(P<0.05)。结论 :5/8基因型携带者血浆Lp(a)水平升高 。 Objective:To investigate the distribution of5pentanucleotide TTTTA repeat polymorphism of apo(a)gene in patients with coronary heart disease(CHD)and healthy controls,and to observe the relationship between polymorphism of apo(a)and serum Lp(a)levels.Methods:5pentanucleotide TTTTA repeat polyˉmorphism of apo(a)gene were analyzed in293patients with CHD and163healthy controls.The corresponding DNA fragments were amplified with PCR,and PCR products were separated by polyacrylamide gel elecˉtrophoresis.Serum Lp(a)levels were measured by ELISA.Results:Eight alleles and19genotypes were found totally.The common genotypes were8/8,8/9,5/8,and9/9,respectively.Serum Lp(a)level was higher in5/8genotype in both patients and controls(P<0.05).The higher distribution of5/8genotype in group with high Lp(a)and CHD group were observed(P<0.05).Conclusion:Serum Lp(a)was related to5/8genotype.Peoˉple with5/8genotype have higher risk for CHD.
作者 程津新 毛用敏 张燕杰 陈倩 崔让庄
机构地区 天津市胸科医院
出处 《天津医药》 CAS 北大核心 2004年第7期385-388,共4页 Tianjin Medical Journal
关键词 载脂蛋白(a) 五核苷酸重复序列基因多态性 冠心病 血浆Lp(a) apolipoproteins A polymorphism(genetics)coronary disease
分类号 R541.4 [医药卫生—心血管疾病]
  • 相关文献

参考文献10

  • 1Luc G, Bard JM, Arveiler D, et al. Lipoprotein(a) as a predictor of coronary heart disease: the PRIME study.Atherosclerosis, 2002, 163:377-384.
  • 2Hoogeveen RC, Gambhir JK, Gambhir DS, et al. Evaluation of Lp(a) and other independent risk factors for CHD in Asian Indians and their USA counterparts. J Lipid Res, 2001, 42:631 - 638.
  • 3Valenti K, Aveynier E, Leaute S, et al. Contribution of apo (a) size, pentanucleotide TTTTA repeat and C/7( + 93) polymorphisms of the apo(a) gene to regulation of lipoprotein(a) plasma levels in a population of young European Caucasians.Atherosclerosis, 1999, 147:17- 24.
  • 4Puckey LH, Lawn RM, Knight BL, et al. Polymorphisms is the apolipoprotein(a) gene and their relationship to allele size and plasma Lp(a) concentration. Hum Mol Gent, 1997, 6:1099- 1107.
  • 5胡波,周新,李林,李玺.冠状动脉粥样硬化性心脏病患者载脂蛋白(a)五核苷酸重复序列基因多态性的初步研究[J].中华医学遗传学杂志,2002,19(1):46-48. 被引量：8
  • 6Alaoui M, Merkec C, Garnotel R, et al. Study of apo(a)length polymorphism and lipoprotein(a) concentration in subjects with single or double apo(a) isoforms. Clln Chem Lab Med, 2003, 41 : 634 - 639.
  • 7Boosmsma DI, Kaptein A, Kempen HJM, et al. Lipoprotein (a) relation to other risk factors and genetic heritability results from a Dutch parent - twin study. Atherosclerosis, 1993, 99:323 - 333.
  • 8Brazier L, Tirec L, Luc G, et al. Sequence polymorphisms in the apo(a) gene and their association with Lp(a) levels and myocardial infarction, The ECTIM Study. Atherosclerosis,1999. 142:323 - 333.
  • 9Kalna A, Csaszar A, Ffist G, et al. The association of serum Lp(a) levels, apo(a) size and (TTTTA)n polymorphism with coronary heart disease. Clin Chim Aeta, 2001,309:45-51.
  • 10Amemiya H, Arenami T, Kikuehi S, et al. Apo(a) size and pentanucleotide repeat polymorphism are associated with degree of atherosclerosis in coronary heart disease. Atherosclerosis, 1996, 123:181 - 191.

二级参考文献10

  • 1Mclean JW, Tomlinson JE, Kuang WJ, et al. cDNA sequence of human apolipoprotein(a) is homologous to plasminogen. Nature, 1987,330∶132-137.
  • 2Yuan BM. The diagnostic criteria of cardiovascular disease. 1st ed. Beijing:Xueyuan Press,1992.178-181. [元柏民. 心血管病诊断标准.第1版.北京:学苑出版社,1992.178-181.]
  • 3Loparev VN, Cantus MA, Monken CE, et al. An efficient and simple method of DNA extraction from whole blood and cell to identify infectious agent. J Virol Methods,1990,34∶105-112.
  • 4Malgaretti N, Acquati F, Magnaghi P, et al. Characterization by yeast artificial chromosome cloning of the linked apolipoprotein(a) and plasminogen genes and identification of the apolipoprotein(a) 5′ flanking region. Proc Natl Acad Sci U S A,1992,89∶11584-11588.
  • 5Woolf B. On estimating the relation between blood group and disease. Ann Hum Genet,1955,19∶251-253.
  • 6Trommsdroff M, Kochl S, Lingenhel A, et al. A pentanucleotide repeat polymorphism in the 5′ control region of the apolipoprotein(a) gene is associated with lipoprotein(a) plasma concentrations in Caucasians. J Clin Invest, 1995, 96∶150-157.
  • 7Rosby O , Berg K. LPA gene:interaction between the apolipoprotein(a) size (kringle Ⅳ repeat) polymorphism and a pentanucleotide repeat polymorphism influences lipoprotein(a) level. J Intern Med,2000,247∶139-152.
  • 8Valenti K, Aveynier E, Leaute S, et al. Contribution of apolipoprotein(a) size, pentanucleotide TTTTA repeat and C/T(+93)polymorphisms of the apo(a) gene to regulation of lipoprotein(a) plasma levels in a population of young European Caucasians. Atherosclerosis,1999,147∶17-24.
  • 9Amemiya H, Arinami T, Kikuchi S, et al. Apolipoprotein(a) size and pentanucleotide repeat polymorphisms are associated with the degree of atherosclerosis in coronary heart disease.Atherosclerosis,1996,123∶181-191.
  • 100 Volkova MV, Vasina VI, Fomicheva EV, et al. Comparative analysis of apo(a) gene alleles: distribution of pentanucleotide repeats in position -1373 and C/T transition in position + 93 among patients with myocardial infarction and a control group in St.Petersburg, Russia. Biochem Mol Med, 1997, 61∶208-213.

共引文献7

同被引文献13

  • 1丁伟,陈沐.无症状脑梗塞病因学及临床分析探讨[J].云南医药,1997,18(2):108-108. 被引量：1
  • 2Hoshi T,Kitagawa K,Yamagami H,et al.Relations of serum high-sensitivity C-reactive protein and interleukin-6 levels with silent brain infarction[J].Stroke,2005,36(5):768.
  • 3Yamagami H,Kitagawa K,Nagai Y,et al.Higher levels of interleukin-6are associated with lower echogenicity of carotid artery plaques[J].Stroke,2004,35 (3):677-681.
  • 4Hashimoto H,Kitagawa K,Hougaku H,et al.C-reactive protein is an independent predictor of the rate of increase in early carotid atherosclerosis[J].Circulation,2001,104(1):63-67.
  • 5Lee SC,Park SJ,Ki HK,et al.Prevalence and risk factors of silent cerebral infarction in apparently normal adults[J].Hypertension,2000,36(1):73-77.
  • 6王鹏 张超 袁永.无症状性脑梗死研究进展[J].中国慢性病预防与控制,1998,144(1):111-112.
  • 7Matsui T,Arai H,Yuzuriha T,et al.Elevated Plasma Homocysteine Levels and Risk of Silent Brain Infarction in Elderly People[J].Stroke,2001,32(2):1116.
  • 8Vermeer SE,Koudstaal PJ,Oudkerk M,et al.Prevalence and risk factors of silent brain infarcts in the population-based rotterdam scan study[J].Stroke,2002,33 (1):21-25.
  • 9Uehara T,Tabuchi M,Mori E.Risk factors for silent cerebral infarcts in subcortical white matter and basal ganglia[J].Stroke,1999,30(3):378-382.
  • 10Howard G,Wagenknecht LE,Cai JW,et al.Cigarette smoking and other risk factors for silent cerebral infarction in the general population[J].Stroke,1998,29(3):913-917.

引证文献1

二级引证文献11

天津医药
2004年 第7期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部