宫颈癌与HPV16和18及VEGF和TGF-β_1关系的探讨

Study the relationship among HPV 16 and 18,VEGF and TGF-β_1 in squamous of cervical cancer

目的 :探讨HPV16、18,VEGF、TGF β1与宫颈鳞癌的发生、发展的关系。方法 :99例宫颈癌患者及 5 4例对照组取患者宫颈分泌物 ,使用荧光基因定量PCR法检测HPV16、18,取宫颈癌组Ⅰ、Ⅱ期HPV16、18阳性患者的鳞癌组织用免疫组化方法进行VEGF、TGF β1检测 ,结果 :宫颈癌组HPV16、18水平明显高于对照组 ,且差异有统计学意义 ,P <0 0 5 ;淋巴结转移组中HPV阳性表达高于HPV阴性表达 ,且差异无统计学意义 ,P >0 0 5 ;VEGF、TGF β1在宫颈鳞癌Ⅰ、Ⅱ期表达呈增高趋势 ,组间比较差异无统计学意义 ,P>0 0 5。结论 :HPV16、18可能为宫颈癌的主要危险因素 ,HPV上调VEGF、TGF β1启动子的活性 ,VEGF可促进肿瘤细胞的增生与转移 ;TGF β1可能与机体免疫功能降低及肿瘤细胞免疫逃逸有关。

OBJECTIVE:To investigate the relationship among HPV16,18,VEGF and TGF-β1 in squamous of uterine cervix.METHODS:Cells exfoliated from cervix.(collected by clinician)of 99 women with cervical cancer and 54 women as a control group,which were analyzed blindly Human papillomavirus Type16 and 18 Fluorescent polymerase Reaction Diagnositic kit.The positive Hpv16,18 of 38 women with squamors of cervical cancer were studied for the expression of VEGF,TGF-β 1by immunohistochemical stain.RESULTS:The positive expression of HPV16,18.was observed in 53 in the case of cervical cancer with positive rates of 53.5%,but in control group the positive rates was 7.3%,P<0.05.There was significant between the two groups.In Lymph node metastasis group,The positive expression of HPV16,18 was higher than the negative expression,P>0.05.The VEGF expression was detected in 29 cases(76.3%) in 38 women which HPV16,18 were positive and TGF-β 1 expression was detected in 31(79.5%) cases.They were also related to clinical stages and were higher as clinical stages increased,P<0.05.It was no significance between clinical stages.CONCLUSIONS:The main risk factor for cervical cancer in Shanxi regiong was Hpv16,18 infection The VEGF expression is positively correlated with the cancer cells proliferation and metastasis;the TGF-β 1 expression may be associated with the decreasing of immunoregulation function and escape from of immunal survellance.