环氧化酶-2蛋白在复发性原发上皮性卵巢癌中的表达

Expression of COX-2 protein in primary epithelia ovarian cancer of chemotherapy resistance

目的 :探讨环氧化酶 -2 (cyclooxygenase 2 ,COX 2 )与上皮性卵巢癌化疗疗效 (耐药或敏感 )及临床病理的关系。方法 :1999年 1月～ 2 0 0 3年 6月 ,从临床资料及随访资料完整的卵巢癌病例中筛选出 60例原发上皮性卵巢癌 (primaryepitheliaovariancarcino ma ,PEOC)作为研究对象。另选取 6例宫颈癌切除术后病理证实双卵巢为正常组织的病例作为对照。根据临床诊断、初治后复发时间等因素将 60例卵巢癌患者分为化疗耐药组 ( 3 0例 )和化疗敏感组 ( 3 0例 )。与 6例正常人作为对照组全部 66例均有随访结果。用流式细胞术定量分析 66例标本组织中COX 2蛋白的表达量。结果 :COX 2蛋白的表达量化疗耐药组 >化疗敏感组 >正常卵巢组织 ,单向方差分析两两比较差异均有统计学意义 ,P <0 0 0 5。随临床分期、病理分级、化疗疗程数的增加及有淋巴结转移 ,COX 2蛋白的表达量也随之增加 ,但是差异无统计学意义 ,P >0 0 5。结论 :COX 2蛋白的表达量与上皮性卵巢癌的复发、发展及疗程有关 ,与化疗耐药呈正相关。

OBJECTIVE:To explore the relation among the expression of COX-2 protein,and curative effect of chemotherapy and clinical pathology in primary epithelia ovarion cancer (PEOC).METHODS:The paraffin-embedded samples of 60 cases with PEOC and 6 cases with normal ovarian were selected from January,1999 to June,2003 were selected.All of them were divided into three groups:1)drug-resistance group (30 cases),2)drug-sensitive group (30 cases),3)normal ovarian group (6 cases,as controls).COX-2 protein were detected respectively in 66 samples by flow cytometry (FCM).RESULTS: The expression of COX-2 protein had significant difference among the three groups,1)>2)>3),P<0.005 .It had no differences among clinical stage,pathology grade,courses of chemotherapy and lymph node metastasis though it had increased with their increasing,P>0.05.CONCLUSION: The expression of COX-2 has positive relation to chemotherapy resistance to PEOC,also has correlation to its recurrence, development and courses of chemotherapy.