胃癌细胞增殖活性与淋巴结转移关系的研究

Selationship between the cellular proliferative activity and lymph node metastasis in gastric carcinoma

目的 探讨溴化脱氧脲嘧啶核苷 (BrdUrd) /DNA双参数流式细胞术 (FCM )检测胃癌的价值 ,研究胃癌细胞BrdUrd标记指数 (LI) ,G2 /M期细胞比率 (G2 /MPF)和DNA含量与胃癌淋巴结转移的关系。方法 60例胃癌新鲜标本 ,用BrdUrd/DNA双参数流式细胞术进行检测。结果淋巴结阳性者 ,BrdurdLI明显增高 (P <0 .0 1,P =0 .0 0 0 5 8) ,G2 /MPF明显增高 (P <0 .0 1,P =0 .0 0 2 70 )。淋巴结转移个数大于 5枚者 ,BrdUrdLI较无转移者明显增高 (P <0 .0 1,P =6.43E 0 5 ) ,较转移 1～ 4枚者明显增高 (P <0 .0 5 ,P =0 .0 43 10 ) ,G2 /MPF较无转移者明显增高(P <0 .0 5 ,P =0 .0 0 840 ) ;淋巴结转移 1～ 4枚者 ,G2 /MPF较无转移者明显增高 (P <0 .0 5 ,P =0 .0 160 0 )。淋巴结转移第Ⅱ站以远者 ,BrdurdLI较无转移者明显增高 (P <0 .0 1,P =0 .0 0 13 2 ) ,G2 /MPF较无转移者明显增高 (P <0 .0 1,P =0 .0 0 13 0 ) ;淋巴结转移第Ⅰ站者 ,G2 /MPF较无转移者明显增高 (P <0 .0 1,P =0 .0 0 718) ,异倍体癌淋巴结阳性者明显增多 (P <0 .0 5 ,χ2 =5 .71)。结论 胃癌细胞BrdUrdLI、G2 /MPF和DNA含量与淋巴结转移及转移数量和距离有关。

Objective To evaluate the value of BrdUrd/DNA doubleparametric method in the detection of gastric carcinoma and to study the relationship between cellular BrdUrd LI,G 2/MPF and DNA content and lymph node metastasis.Methods Sixty cases of fresh tumor samples were examined by BrdUrd/DNA doubleparametric flow cytometry.Results BrdUrd LI and G 2/MPF values with lymph node metastasis were higher than those without metastasis ( P <0.01,P =0.000 58,0.002 70);The BrdUrd LI and G 2/MPF values were correlated with the number of lymph node metastasis;BrdUrd LI with 5 metastatic lymph nodes was higher than those with 1-4 metastatic lymph nodes ( P <0.05,P = 0.043?10) and no metastasis ( P <0.01, P =6.43E-05).G 2/MPF values with 5 and 1-4 metastatic lymph nodes were higher than those without metastasis ( P <0.01,P =0.008?40) and P <0.05,P = 0.016?00). BrdUrd LI and G 2/MPF values were both significantly higher in lymph metastasis to N2↑than those without metastasis ( P <0.01,P =0.001?32,P =0.001?30);G 2/MPF values were significantly higher in lymph metastasis to N1 than those without metastasis ( P <0.01,P =0.007?18);The incidence of lymph node metastasis was significantly higher in aneuploid carcinoma ( P <0.05,χ 2= 5.71). Conclusion Correlations between cellular BrdUrd LI,G 2/MPF,DNA content and lymph node metastasis exist in gastric carcinoma.