血管内皮生长因子-C在胃癌中的表达及其与淋巴结转移的关系

Expression of vascular endothelial growth factor C and its correlation with lymph node metastasis in gastric carcinoma

目的 研究血管内皮生长因子 C(VEGF C)在胃癌中的表达并探讨其与淋巴结转移的关系。方法 应用逆转录 聚合酶链反应 (RT PCR)检测VEGF CmRNA在 5株胃癌细胞株中的表达情况 ,同时采用免疫组织化学法 ,检测 63例接受根治性切除手术病例的胃癌组织标本VEGF C蛋白的表达。结果 VEGF CmRNA表达于胃癌细胞株MKN 45、SGC 790 1及AGS。VEGF C蛋白则在 5 2 .4%(3 3 /63 )的病例中呈阳性表达。在伴淋巴结转移的胃癌中 ,VEGF C表达较无淋巴结转移者更显著 (P <0 .0 1)。同时 ,VEGF C表达与淋巴管浸润和TNM分期密切相关 (P <0 .0 1) ,但与年龄、性别、肿瘤大小、位置、Lauren分型、浸润深度和血管浸润均无明显相关。结论 VEGF C的表达与胃癌淋巴结转移密切相关。

Objective To study the expression of vascular endothelial growth factor C (VEGF-C) in gastric carcinoma and its relationship with lymph node metastasis.Methods The expression of VEGF-C mRNA in 5 gastric carcinoma cell lines was examined by reverse transcription-polymerase chain reaction (RT-PCR).Simultaneously,the expression of VEGF-C protein in gastric carcinoma tissues obtained from 63 patients who underwent radical gastrectomy was detected by immunohistochemistry.Results Three of the 5 gastric carcinoma cell lines,including MKN-45,SGC-7901 and AGS,expressed VEGF-C mRNA.VEGF-C protein was expressed in 52.4% (33/63) of patients.VEGF-C protein expression was more frequently detected in tumors with lymph node metastasis than in those without ( P <0.01).VEGF-C protein expression was also closely related to lymphatic invasion ( P <0.01) and TNM stage ( P <0.01).However,there was no significant correlation between VEGF-C expression and the age,gender,tumor size,tumor location,Lauren classification,depth of invasion,and vascular invasion.Conclusion The expression of VEGF-C is closely related to lymph node metastasis of gastric carcinoma.