荧光定量PCR检测慢性乙肝患者外周血单个核细胞乙肝病毒DNA

Detection of HBV DNA in Peripheral Blood Mononuclear Cells with Fluorescent Quantitative PCR in Patients with Chronic Hepatitis B

目的 探讨定量检测慢性乙肝患者外周血单个核细胞 (PBMC)乙型肝炎病毒DNA(HBV -DNA)的临床意义。方法 采用荧光定量PCR检测PBMC及血清中HBV -DNA含量。结果 49例慢性乙肝患者PBMC及血清中HBV -DNA阳性率分别为44 9%( 2 2 /4 9) ,5 1 0 %( 2 5 /4 9) ,两者阳性率无统计学意义的差异 (P >0 0 5 ) ,具有一致性 ;血清HBV -DNA高水平组PBMC的HBV-DNA含量与低水平组比较 ,两者有统计学意义差异 (P <0 0 1) ;血清HBV -DNA高水平组PBMC的HBV -DNA阳性率 ( 10 0 %)与血清低水平组PBMC的HBV -DNA阳性率 ( 4 2 9%)比较 ,两者有统计学意义的差异 (P <0 0 5 ) ;在 2 4例血清HBV -DNA阴性患者中 ,发现 5例PBMCHBV -DNA阳性。结论 PBMC的HBV -DNA检测可反映病毒在体内的复制程度 ,是对慢乙肝患者血清HBV -DNA检测有意义的补充 。

Objective To study the clinical significance of quantitative detection of HBV DNA in peripheral blood mononuclear cells (PBMC) of the patients with chronic hepatitis B. Methods HBV-DNA in PBMC and serum of the patients with chronic hepatitis B was detected by fluorescent quantitative PCR (FQ-PCR) technology. Results In 49 patients with chronic hepatitis B, the positive rate of HBV-DNA in PBMC and serum was 44 9%(22/49), 51 0%(25/49) respectively, which had not significant significance (P>0 05). Between the patients with high level of serum HBV-DNA and the ones with low level of serum HBV-DNA, both the quantity and positive rate of HBV-DNA in PBMC had a significant difference (P<0 01,P<0 05). Conclusion The results suggested that the detection of HBV-DNA in PBMC can play a complementary role for monitoring the state of HBV infection and replication.