共刺激分子B7和CD40对日本血吸虫感染小鼠Th1/Th2免疫偏移的调控作用

Effects of B7 and CD40 Costimulatory Molecules on Th1/Th2 Immune Deviation of Mice Infected with Schistosoma japonicum

为观察干预B7 CD2 8和CD4 0 CD4 0L共刺激信号对Th1 Th2细胞因子表达水平和Th1 Th2免疫偏移的调控作用 ,分别取小鼠感染日本血吸虫后 6、8、1 0和 1 2周的脾淋巴细胞经抗CD80 (B7 1 )mAb、抗CD86 (B7 2 )mAb、抗CD4 0mAb和抗CD4 0LmAb处理后培养 72h ,用ELISA双抗体夹心法测定培养上清中IFN γ和IL 4的表达水平的动态变化并分析干预 2种不同的共刺激信号对Th1 Th2免疫偏移的影响。结果显示抗CD80mAb和抗CD86mAb均能显著抑制IL 4的表达水平 ,尤其是抗CD86mAb对IL 4抑制作用尤为明显。抗CD4 0mAb和抗CD4 0LmAb也能影响Th2细胞因子的表达。其中以阻断CD4 0分子的作用更为显著。结果提示B7 CD2 8和CD4 0 CD4 0L共刺激信号可以调节Th1 Th2细胞因子的表达水平和调控Th1 Th2免疫偏移。干预B7 CD2 8和CD4 0 CD4 0L介导的共刺激信号调控Th1

To observe the effect of B7 and CD40 costimulatory molecules on Th1/Th2 cytokine expression levels and immune deviation in the mice infected with Schistosoma japonicum . Murine spleen lymphocyte cultures were made at 6, 8, 10 and 12 weeks after infection of the mice infected with Schistosoma japonicum. The cultures were incubated with anti CD80mAb, anti CD86mAb, anti CD40mAb and anti CD40LmAb for 72?h. The kits of ELISA were used for the quantitative determination IFN γ and IL 4 in the spleen lymphocyte culture medium. The results of analysis by the Th2 differentiation index that used to evaluate the immune balance of Th1/Th2 cells. The results showed that anti CD80mAb and anti CD86mAb can significantly inhibit the expression of IL 4 ( p <0.01),and the effect of anti CD86mAb is more significant than that of anti CD80mAb. Anti CD40mAb and anti CD40LmAb can also significantly inhibit the expression of IL 4 ( p <0.01),and the effect of anti CD40mAb is more significant than that of anti CD40LmAb. These findings suggest that the interference of costimulatory signals can modulate Th1/Th2 cytokine expression levels and immune deviation in the mice infected with Schistosoma japonicum. A novel potential approach is to control the egg granuloma formation of Schistosoma japonicum by B7 or CD40 as a mediator of Th1/Th2 immune deviation.