中药益智胶囊对大鼠全脑缺血后海马CA1区神经元迟发性死亡的影响

Effects of yizhi capsule, a preparation of traditional Chinese medicines, on delayed neuronal death in hippocampal CA1 region and memory function of rats after ischemia-reperfusion injury

目的 观察中药益智胶囊对大鼠全脑缺血后海马CA1区神经元迟发性死亡及学习功能的影响。方法 以四血管闭塞(4-VO)法制成大鼠急性全脑缺血再灌注模型。采用免疫组织化学方法计数脑缺血再灌注脑损伤后1、2、4、8及40d后,大鼠海马CA1区正常神经元数目;用Morris水迷宫实验观察脑缺血再灌注脑损伤后40d时各组大鼠的学习记忆功能。结果 从缺血再灌注后第2天起,益智胶囊(100mg/kg·b.w.)组大鼠海马CA1区正常神经元数量显著高多于缺血对照组大鼠(P<0.01)。全脑缺血再灌注脑损伤后40d时,益智胶囊(100mg/kg·b.w.)组大鼠记忆功能明显好于缺血对照组大鼠(P<0.01)。结论 益智胶囊对全脑缺血后大鼠海马CA1区神经元具有保护作用,并可改善大鼠的记忆功能障碍。

Objective To observe the effects of Yizhi capsule, a preparation of traditional Chinese drugs on delayed neuronal death in the hippocampal CA1 region and memory function of rats after ischemia-reperfusion injury. Methods Rat models of acute reperfusion injury after global cerebral iscbemia were induced by vertebral and carotid arteries occlusion, and 1, 2, 4, 8 and 40 d after model establishment, the neurons in the rat hippocampal CA1 region were obtained and immunohistochemically stained for counting. Morris water maze test was performed to observe the learning and memory capacities of the rats 40 d after the injury. Results The number of normal neurons was significantly higher in the rats treated with Yizhi capsule (100 mg/kg·b.w.) than in those without the treatment, and the former group of rats used significantly shorter time in finding the platform under the water surface in Morris water maze test. Conclusion Yizhi capsule may protect the neurons in the hippocampal CA1 region and improve on the learning and memory dysfunction after global ischemia/reperfusion injury in rats.