蜕膜细胞条件培养液对卵巢癌细胞株COCl侵袭相关基因表达的影响

Effect of decidual cell conditioned media on invasion-related gene expression of ovarian tumor cell line COC1

目的 研究蜕膜细胞条件培养液(DCM)对卵巢癌相关侵袭基因表达的影响。方法 原代培养早孕蜕膜细胞及晚孕蜕膜细胞并提取DCM,用DCM处理卵巢癌细胞株COCl,利用RT-PCR法对卵巢癌侵袭相关基因的表达进行分析。结果卵巢癌细胞株COC1表达基质金属蛋白酶2(MMP-2)、基质金属蛋白酶抑制剂(TIMP-2)及纤溶酶原抑制剂(PAI-1),而不表达MMP-9、TIMP-1及尿激酶型纤溶酶原激活物(u-PA)。早孕及晚孕DCM能显著下调卵巢癌细胞株COC1 MMP-2的表达(P<0.01);早孕及晚孕DCM处理的卵巢癌细胞株COC1也表达TIMP-2、PAI-1 mRNA,且有显著差异(P<0.01)。结论DCM可以通过改变卵巢癌细胞株COCl侵袭基因MMP-2/TIMP-2以及B-PA/PAI-1之间的平衡,从而降低卵巢癌细胞株COCl的侵袭能力。

Objective To study the effect of decidual cell conditioned media (DCM) on the expression of the invasion-related gene of ovarian tumor cell line COC1. Methods After primary culture of the decidual cells of early and late pregnancy, DCM was extracted from the cell cultures for treatment of the ovarian tumor cell line COC1. Analysis of the invasion-related gene expression in COC1 cells was performed by way of reverse transcriptional (RT)-PCR. Results The COC1 expressed the mRNAs of matrix metalloproteinase 2 (MMP-2), tissue inhibitor of metalloproteinase-2 (TIMP-2) and plasminogen activator inhibitor type 1 (PAI-1), but not those of MMP-9, TIMP-1 or urokinase-type plasminogen activator (u-PA). The DCM of the early- and late-pregnancy decidual cell cultures significantly down-regulated the MMP-2 expression, and up-regulated the expression of the TIMP-2 and PAI-1 mRNA in COC1 cells in comparison with the control cells (P<0.01). Conclusion DCM can lower the invasive capacity of the ovarian tumor cell line COC1 by interrupting the balance between MMP-2 and TIMP-2 and between u-PA and PAI-1.