妊娠期接受MRI磁场照射对子代大鼠不同发育期MAPK的影响

Effects of prenatal exposure of rats to MRI magnetic fields on protein expression of MAPK at different stages of postnatal development

【目的】 研究妊娠期接受磁共振 (magneticresonanceimaging ,MRI)复合磁场照射对子代大鼠不同发育期脑部丝裂原活化蛋白激酶 (mitogenactivatedproteinkinase ,MAPK) /细胞外信号调节蛋白激酶 (extracellularsignalregulatedproteinkinase ,ERK )表达的影响。 【方法】 SD孕鼠妊娠第 12～ 18d给予 0 .3 5TMRI磁场照射 ,雌性子鼠1、2、5月龄时进行大脑切片免疫组化SP方法染色 ,并用图像分析仪进行半定量分析。 【结果】 MAPK/ERK免疫反应在大鼠脑切片上广泛表达 ;随着年龄增长 ,未发现其表达的变化 ;在各年龄段子鼠照射组和对照组间MAPK/ERK的免疫反应表达差异均无显著性 (P >0 .0 5 )。

To investigate the effects of prenatal exposure to the magnetic resonance imaging (MRI) magnetic fields on protein expression of mitogen activated protein kinase (MAPK)at different stages of postnatal development. Pregnant rats on gestation of the 12 th ～18 th days were exposed to the magnetic fields used for MRI applications. When the female offspring were 1, 2, and 5 month old, the SP immunohistochemical staining was made in the paraformaldehyde fixed hippocampal sections. Computer assisted image was taken for analysis. There was a widespread expression of MAPK/ERK (extracellular signal regulated protein kinase)in the brain sections. No significant effect was detected along with development. No differences of all the ages were observed between MRI and control groups in MAPK/ERK immunoreactivity. [Conclusion] Prenatal exposure to the MRI magnetic field did not induce changes of MAPK/ERK immunoreactivity.