肠内营养对长期禁食病人结肠黏膜增生状态的影响

Effect of enteral nutrition on the proliferation of colon mucosa in long fasting patients

目的 :研究肠内营养对病人长期禁食状况下结肠黏膜增生状态的影响。 方法 :选择禁食时间超过 2周以上的病人 1 0例。在禁食状态和给予肠内营养 2周后 ,分别经纤维结肠镜获取结肠黏膜 ,作流式细胞计数检查。根据流式细胞仪计数结肠黏膜上皮细胞周期G0 ～G1 %、G2 M %、S %和凋亡 (Apto)的百分比 ,通过公式计算得出增生指数 (PI)和S期细胞比率 (SPF) ,作为评估结肠黏膜上皮细胞增生状态的指标。 结果 :在长期禁食状态下 ,结肠黏膜上皮细胞增生情况分别为 :PI为 (4 .0 1 0± 1 .2 90 ) % ;SPF为 (1 .90 4± 2 .6 79) % ;Apto为 (1 .91 3± 2 .0 2 1 ) %。肠内营养 2周后 ,结肠黏膜上皮细胞的增生情况 :PI为 (7.337± 2 .6 79) % (与禁食状态相比较 ,P <0 .0 1 ) ;SPF为(5 .36 7± 3.2 1 1 ) % (与禁食状态相比较 ,P <0 .0 1 ) ;Apto为 (1 .2 0 8± 1 .4 88) %。 结论 :长期禁食病人 ,结肠黏膜上皮细胞增生处于低下状态。肠内营养 2周后 。

Objective: To investigate the effect of enteral nutrition on proliferation of colon mucusa in long fasting patients. Methods: Ten patients fasted over two weeks were selected.All patients had no any histories of chemotherapy, radiotherapy and immunosuppressive therapy.They received enteral nutrition for tow weeks. Before and two weeks after enteral nutrition,specimens were obtained between sigmoid and descending colon by colonofibroscope.All specimens of colon mucosa were determined by FCM for proliferation index (PI),S phase factor (SPF)and aptosis(Apto). Results: PI,SPF and Apto were ( 4.010 ±1.290)%,(1.904±2.679)% and (1.913±2.021)% in patients fasted over two weeks respectively, and (7.337±2.679)%,(5.367±3.211)% and (1.208±1.488)% after enteral nutrition for two weeks. Conclusions: Enteral nutrition could improve the proliferation of colon mucosa in long fasting patients.