摘要
目的建立益肝凉茶中栀子苷的含量测定方法。方法采用RP-HPLC法,LunaC18色谱柱,乙腈-水(15∶85)为流动相;流速为1.0mL·min-1;检测波长为238nm进行测定。结果栀子苷线性范围为0.2024μg～1.0120μg,r=1.000;平均回收率为98.1%,RSD值为0.42%(n=5)。结论该方法灵敏度高、操作简便、结果准确,可用于益肝凉茶的质量控制。
Objective :To establish a RP-HPLC method for the determination geniposide in Yigan Liangcha.Methods Luna C18 (4.6 mm×250 mm,5 μm)was applied,the mobile phases consisted of acetonitrile-water(15∶85),the flow rate was 1.0 mL·min-1 and the detection wavelength at 238 nm.Results The average recovery of geniposide was 98.1 %and RSD were 0.42 %(n=5).There is a good linear relationship within the range of 10.12～50.60 μg of geniposide.Conclusion The method is convenient,sensitive,accurate and reproducible and can be used the quality control of the Yigan Liangcha.
出处
《中药新药与临床药理》
CAS
CSCD
2004年第4期274-276,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
