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家蚕胚胎和幼虫时期滞育激素的组织学定位、表达和分泌路径 被引量:4

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摘要 利用原位杂交和免疫组化方法对家蚕滞育激素(DH)基因在胚胎时期神经系统中的分布、转录水平和翻译水平的表达进行了研究.免疫组化结果表明,胚胎的整个中枢神经系统中,包括脑、咽下神经节和胸神经节(TG),都有明显的阳性细胞;心侧体和末端腹神经节(TAG)很可能是DH的释放位点;以地高辛标记的DHcRNA为探针的原位杂交结果表明,DHmRNA在胚胎咽下神经节的上颚、下颚和上唇3簇细胞中表达.此外,发现胚胎的咽下神经节的一对侧边细胞和TG的一对腹中线细胞也表达DHmRNA.原位杂交和RT-PCR的结果表明5龄幼虫期的DHmRNA的表达定位与胚胎期基本一致.
出处 《科学通报》 EI CAS CSCD 北大核心 2004年第12期1151-1156,共6页 Chinese Science Bulletin
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