利用Labworks凝胶成像分析系统判断甲肝减毒活疫苗RT-PCR滴度

Applying Labworks Image Acquisition and Analysis Software for Judging the Titer of HAV Live Attenuated Vaccine

目的 利用Labworks凝胶成像分析系统判断甲肝减毒活疫苗RT PCR滴度 ,并比较此方法与常规组织培养法的相关性。方法 通过对RT PCR反应液的离子浓度、pH值等条件进行优化 ,建立了检测甲肝减毒活疫苗滴度的RT PCR一步法 ;利用Labworks凝胶成像分析系统的半定量功能判断结果 ,并与常规组织培养法比较。结果 利用Labworks凝胶成像分析系统判断结果的RT PCR一步法与常规组织培养法检测甲肝减毒活疫苗滴度灵敏度相仿 ,检测结果无显著差异。结论 此法与常规RT PCR法相比具有更简便 ,判断标准更客观的优点 ,可代替组织培养法检测甲肝减毒活疫苗滴度。

Objective By applying labworks image acquisition and analysis software, to measure the titer of HAV live attenuated vaccine, and to analyze the relationship between this method and routine method (cell culture infective dose 50 percent (CCID50)). Methods The one-step RT-PCR was adopted to detect the titer of HAV live attenuated vaccine, the half-quantitative function of labworks image acquisition and analysis software was applied to measure the outcomes. The results were compared with those obtained by CCID50. Results There was no significant difference in the sensitivity between the above method and traditional method. Conclusions The above method is more convenient and objective compared with routine RT-PCR, which can be used to measure the titer of HAV live attenuated vaccine.