酶联免疫吸附试验检测姜片虫感染者血清抗体

Studies on detecting antibodies in human sera of Fasciolopsis buski by ELISA

超声粉碎制备姜片吸虫成虫冷浸抗原,以1:3500工作浓度包被酶标板;采用Kato—Katz氏定透法普查姜片吸虫病,对虫卵阳性者用ELISA法测定血清抗体,同时测定健康居民的血清和其他吸虫病患者血清,以评价ELISA法检测姜片虫病血清抗体的敏感性、特异性和交叉反应性。按常规ELISA方法操作,目视与酶标仪判断结果。普查2 189人中姜片吸虫卵阳性者168例;168例中165例ELISA阳性,ELISA与Kato—Katz氏定透法的阳性符合率98.21％;健康居民血清147份,阳性6份,阳性率4.08％;与血吸虫病交叉阳性为9.38％,肺吸虫病交叉阳性为5.36％。应用此法检测姜片虫感染者血清抗体具有敏感性高、特异性强和交叉反应率低等特点,可代替粪检法广泛用于姜片吸虫感染的检测。

Methods ELISA plates were coated with the frozen soaked adult antigen of Fasciolopsis butki by ultrasonic shatter at concentration of 1:3,500. A survey for persons with Fasciolopsis buski was carried in Huangshi city by Kato-Katz's thick smear. 168 cases of Fasciolopsis were detected for antibodies against Fasciolopsis buski in sera by ELISA, and a comparative investigation was done in the local residents without Fasciolopsis buski and 96 cases of schistosomiasis japonica and 56 cases of Paragonimus westennani by ELISA for assessment of sensitivity, specificity and crossing reaction. The results were observed with naked eyes and ELISA OD value. Results 165 cases were positive in antibodies against Fasciolopsis buski in human sera among 168 victims of Fasciolopsis buski. The positive correspondence rate between ELISA and Kato-Katz's thick smear was 98.21%. 6 of 147 pieces of sera from the local residents without Fasciolopsis buski was positive in fasci-olopsis antibodies. Positive rate was 4.08%. The crossing positive rate to schistosomiasis was 9.38%, to paragordmiasis was 5.36%. Conclusion ELISA with the frozen soaked adult antigen of Fasciolopsis buski to detect Fasciolopsis antibodies in human sera had the characteristics of high sensitivity, strong specificity and low crossing reaction rate. It could be used to substitute fecal examination techniques for diagnosis of Fasciolopsis buski.