摘要
1986～1990年以圆球草莓品种为试材,对茎尖培养、药剂处理、热处理和花药培养4种脱毒方法进行比较,结果表明,花药培养效果最好,脱毒率100%,茎尖切取长度需小于0.5mm 才能达到20%以上脱毒效果,用较大材料接种培养成试管苗,再用38°±1℃温度处理2周后,切取小茎尖培养能提高脱毒效果,茎尖培养脱毒必须经过病毒鉴定,方能用于生产。
From 1986 to 1990,variety Yuanqiu was used as experimental material to compare 4kinds of virus-elimination techniques.The results showed that the effect of anther culture wasthe best:virus-elimination rate was 100% and that of tip-shoot culture could reach 20% ifthe length of explant was less than 0.5mm.Bigger material was used to induce shoots in vitroand then treated at 38°±1℃ for two weeks,the virus elimination could be improved bysubculturing the treated tips.These treated materials can be applied in practical productiononly after viruses are identified as eliminated.
关键词
草莓
组织培养
脱毒
strawberry
tissue culture
virus-elimination
