葛根素对胰岛素抵抗大鼠脂肪组织中蛋白激酶B表达的影响

Effects of puerarin on protein expression of protein kinase B in adipose tissue of rats fed by high-fat-diet

目的 :在高脂饮食诱导胰岛素抵抗的基础上 ,观察葛根素对胰岛素抵抗大鼠脂肪组织中蛋白激酶B蛋白 (PKB)表达的影响。方法 :选取雄性Wistar大鼠 30只 ,随机分为正常对照组 10只 ,给予基础饲料 ;模型组 2 0只 ,给予高脂饲料。模型组大鼠给予高脂喂养 4周后 ,随机分为 2组 :胰岛素抵抗组 ,继续高脂饮食 ;葛根素治疗组 ,继续高脂饮食同时 ,腹腔注射葛根素注射液 (10 0mg·kg-1·d-1)。干预 6周后 ,蛋白印迹法检测大鼠脂肪组织中胰岛素刺激PKB的蛋白表达含量。结果 :4周后 ,高脂喂养鼠的体重、空腹血糖、胰岛素、甘油三脂、胆固醇及胰岛素抵抗指数 (HOMA IR)明显升高 ,胰岛素敏感指数(ISI)显著下降 ,出现了胰岛素抵抗。葛根素治疗 6周 ,大鼠的血糖、胰岛素及HOMA IR下降 ,ISI显著升高。胰岛素抵抗组大鼠脂肪组织中PKB的表达明显减少 ,较对照组下降了 2 3.5 % (P <0 .0 1)。葛根素治疗 6周后 ,大鼠PKB蛋白表达明显升高 ,较胰岛素抵抗组增加了 18.7% (P <0 .0 1)。结论 :葛根素明显改善胰岛素抵抗 。

AIM: To study the effects of puerarin on protein expression of protein kinase B (PKB) in adipose tissue of rats fed by high-fat-diet. METHODS: 30 male Wistar rats were randomly divided into the control group (n=10) given basic diet, and the model group (n=20) given fat-rich-diet. After 4 weeks, the model group was randomly divided into 2 subgroups: (1) insulin resistant group was continually given high fat-rich-diet; (2) puerarin-treated group accepted high-fat diet and abdominal injection with puerarin injection (100 mg·kg -1·d -1). After 6 week- treatment, the protein expression of PKB stimulated by insulin in adipose tissue was determined with Western blotting at the end of the experiment. RESULTS: The model group showed hyperglycemia, hyperinsulinism, hypertriglyceride, hypercholesterolemia and elevated HOMA insulin resistance index (HOMA-IR), while the insulin resistance index (ISI) was obviously reduced compared to the control group, indicating that the insulin resistant model was induced in this way. Puerarin treatment decreased fasting blood glucose and insulin, which was accompanied by lower HOMA-IR and elevated ISI. Because of long term high fat diet, expression of PKB decreased 23.5% in adipose tissue of the insulin resistant group(P< 0.01)compare with the control group. There was an increase of 18.7%(P< 0.01)in PKB expression of the puerarin-treated group compared to the insulin resistant group. CONCLUSION: Puerarin treatment can significantly elevate expression of PKB and ameliorated the stated of insulin resistance.