巴西蘑菇多糖对小鼠脑组织表达粒-巨噬细胞集落刺激因子的影响

Study on GM-CSF expression in mouse brain tissue induced by agaricus blazei polysaccharide

目的 :研究巴西蘑菇多糖 (ABPS)对小鼠脑组织表达粒 巨噬细胞集落刺激因子 (GM CSF)的影响。方法 :应用组织培养技术、克隆形成法、逆转录 聚合酶链反应 (RT PCR)等方法检测小鼠脑组织在ABPS作用下表达GM CSF的变化。结果 :不同浓度的ABPS对小鼠脑组织表达GM CSF有明显的促进作用 ,浓度范围为 2～ 6mg·ml-1之间 ,最佳浓度为4mg·ml-1。经LightFramesoftware分析 ,小鼠GM CSF在 96 0bp处出现一高峰。结论 :ABPS有促进小鼠脑组织表达GM CSF的作用 。

AIM: To identify the molecular mechanisms of GM-CSF expression in mouse brain tissue after stimulation with different concentration of Agaricus Blazei polysaccharide(ABPS). METHODS: The tissue culture technique, GM-CFU clone forming test, and RT-PCR were used to detect GM-CSF expression in mouse brain tissue which treated by ABPS. RESULTS: GM-CSF expression induced by ABPS obviously increased in mouse brain tissue. The appropriate concentration was 4 g·L -1. The maximum value showed 960 base pairs analyzed by LightFrame Software. CONCLUSION: ABPS can promote the expression of GM-CSF and facilitate CFU-GM expression in mouse brain tissue.