摘要
目的 :探讨不同剂量卡维地络 (CAR)对压力超负荷心肌肥厚幼鼠心肌细胞凋亡的影响 ,以及CAR治疗充血性心力衰竭 (CHF)的作用机制。方法 :建立腹主动脉狭窄所致压力负荷增加心肌肥厚幼鼠模型 ,术后 1周开始给予小剂量CAR(0 .1mg·kg-1·d-1)和大剂量CAR(10mg·kg-1·d-1)干预 7周 ,观察不同剂量CAR对幼鼠血流动力学参数、心肌细胞凋亡、Bcl 2和P5 3蛋白水平表达的影响。结果 :CAR可改善心功能指标 ,降低心肌细胞凋亡指数、上调Bcl 2蛋白及下调P5 3蛋白表达 ,HCAR组作用明显。结论 :CAR可有效减少压力超负荷心肌肥厚幼鼠的心肌细胞凋亡 ,防治CHF ,效果以大剂量明显 。
AIM: To study the effects and mechanism of carvedilol (CAR) at different doses on cardiac myocyte apoptosis in rats with myocardial hypertrophy of CHF. METHODS: Using the animal model of CHF, induced by abdominal aortic constriction in male Wistar rats, hemodyanmics parameters, cardiac myocyte apoptosis, and the expression of Bcl-2 and P53 were investigated in the untreated experimental group (CHF group) at 1 week after operation and treated experimental groups in which rats were treated with CAR at a lower dose (LCAR group, 0.1 mg·kg -1·d -1) and at a higher dose (HCAR group, 10 mg·kg -1·d -1) for 7 weeks since 1 week after operation. The sham-operated rats were as controls (SH group, n=8). RESULTS: Either doses improved heart function and decreased apoptosis index in rats with CHF. The number of myocytes apoptosis and the level of Bcl-2 were significantly higher and P53 was markedly lower in HCAR group than those in LCAR and MCAR groups. CONCLUSION: CAR can effectively decrease myocardiocyte apoptosis, prevent and cure CHF. The effects are dose-dependent, associated with changes of the expression of apoptosis-associated gene.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2004年第7期824-827,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics